EPO对心肺复苏后大鼠海马神经元的细胞凋亡、Bcl-2及Bax表达的影响

Effect of Erythropoietin on neuronal apoptosis,Bcl-2 and Bax expression in Hippocampal Neurons of Rats after Cardiopulmonary Resuscitation

目的观察促红细胞生成素(EPO)在心肺复苏后对大鼠脑海马神经元细胞凋亡、Bcl-2和Bax表达的影响。方法雄性Wistar大鼠32只,随机分4组:正常对照组(A组)、假手术组(B组)、心肺复苏模型组(C组)、EPO干预组(D组)。C组、D组采用窒息的方法制作大鼠呼吸心跳骤停模型,后行心肺复苏治疗(CPR),D组CRP同时静脉给予EPO 3000U/kg;B组仅静脉给予等剂量生理盐水,4小时的脑海马组织,采用免疫组化法观察各组神经元细胞凋亡、Bcl-2及Bax的表达情况。结果光镜下A、B组未见凋亡细胞,C组可见较多凋亡细胞,D组凋亡细胞数少于C组(P<0.01)。A、B组少量Bcl-2、Bax阳性细胞,C、D组Bcl-2、Bax阳性细胞数均高于A、B组(P<0.01),D组Bcl-2阳性细胞数高于C组(P<0.01),D组Bax阳性细胞数低于C组。结论心肺复苏后大鼠海马神经元的细胞凋亡明显增加,Bcl-2、Bax表达上调。EPO可抑制心肺复苏后海马神经元的细胞凋亡、上调Bcl-2表达同时下调Bax表达。

Objective To observe the effects of erythropoietin（EPO） on the hippoeampal neurons apoptotic cells, Bcl-2 and Bax expression in Hippocampal Neurons of Rats after Cardiopulmonary Resuscitation. Methods Thirty-two Wistar rats were randomly divided into four groups： control group, sham operation group,CPR group ,CPR plus EPO group. Cardiac respiratory arrest model were made by apnea method,then gave CPR treatment. EPO group received an intravenous injection of EPO（3000units/kg bodyweight） at very onset of cardiopulmonary resuscitation, when sham op eration group and CPR group received same doses of saline. Expression of apoptotic cell apoptosis , Bel 2 and Bax expression in hippocampal neurons were observed by immunohistochemisitry method. Results Apoptosis was observed by light microscopy no TUNEL positive cell was founded in A,B group . The apoptotic cells more visible in group C,which was less than the number of apoptotic cells group D. A few visible Bcl-2, Bax positive cells was was founded in A, B group . Compared with A,B group the number of Bcl-2,Bax positive ceils in group C was increased （P〈0.01）. The number of Bcl-2 positive cells in group D higher than group C （P〈0.01）. The number of Bax positive cells in group D below than group C （P〈0.01） . Conclusion The number of apoptotic cell increased significantly while the expres- sion of Bcl 2 and Bax was increased after cardiopulmonary resuscitation. EPO could inhibit apoptosis of hippocampal neurons after CPR in rats,up-regulating expression of bcl-2 and down-regulating of bax expression.