摘要
目的探讨慢性乙型肝炎感染者血清乙型肝炎病毒大蛋白(hepatitis B virus large protein,HBV-LP)检测的临床应用价值。方法选择慢性乙型肝炎病毒(HBV)感染者180例,其中HBV-DNA阳性患者130例,HBV-DNA阴性患者50例,采用酶联免疫吸附试验(ELISA)检测HBV-LP、乙肝二对半(HBV-M)、前S1抗原(PreS1Ag)和前S2抗原(PreS2Ag),采用实时荧光定量PCR技术检测HBV-DNA,采用日立7600生化分析仪检测患者肝功能指标。结果 180例慢性HBV感染者中,HBV-LP与HBV-DNA阳性率差异无统计学意义(P>0.05),HBV-LP表达与HBV-DNA水平呈正相关关系(r=0.53,P<0.05);不同二对半模式的慢性HBV感染者,HBV-LP与HBV-DNA阳性率差异,无统计学意义(P>0.05);在慢性HBV感染者中,HBV-LP阳性率高于PreS1Ag(P<0.01);HBV-LP阳性慢性HBV感染者ALT、AST水平高于HBV-LP阴性感染者(P<0.05)。结论 HBV-LP表达与病毒复制有关,并参与对肝细胞的损伤。
Objective To explore the clinical value of detecting hepatitis B virus large protein （HBV-LP） in patients with chronic HBV infection. Methods To select 180 cases with chronic HBV infection including 150 cases with positive HBV-DNA and 50 cases with negative HBV-DNA,hepatitis B virus large protein,five items of hepatitis B,PreS1 and PreS2 antigen were tested by enzyme linked immunosorbent assay, HBV-DNA was quantitatively determined by realtime polymerase chain reaction,and Hepatic function was tested by Hitachi 7600 biochemistry analysator. Results In 180 cases with chronic HBV infection, the positive rate of HBV-LP and HBV-DNA did not differ significantly （P〉0.05）, HBV-LP and HBV-DNA were direct correlation （r=0.53,P〈0.05）. The positive rate of HBV-LP and HBV- DNA did not differ significantly in patients with chronic HBV infection following different mode of five items of hepatitis B （P〉0.05）. The positive rate of HBV-LP was significantly higher than that of PreS1Ag （P〈0.01）. The level of ALT and AST in chronic infected patients with positive HBV-LP were significantly higher that of patients with negative HBV-LP. Conclusion The expression of HBV-LP is relevant with virus replication and hepatocellular injury.
出处
《中国实验诊断学》
2012年第6期1023-1025,共3页
Chinese Journal of Laboratory Diagnosis
基金
阜阳市卫生局科研基金(2010Y010)
