乙型肝炎患者外周血T淋巴细胞亚群比例和Th1/Th2细胞因子水平检测及其临床意义

Detection of proportions of lymphocyte T subsets in peripheral blood and levels of Th1/Th2 cytokines in patients with hepatitis B virus infection and its clinical significance

目的:研究各型乙型肝炎患者和健康者外周血T淋巴细胞亚群和Th1/Th2细胞因子水平变化及其临床意义,为诊断、治疗疾病提供实验依据。方法:采用流式细胞仪检测34例乙型肝炎患者(其中慢性乙型肝炎中度组14例,慢性乙型肝炎重度组7例,重型乙型肝炎组8例,急性乙型肝炎组5例)和20例健康对照者(对照组)的外周血CD3+、CD4+和CD8+T细胞占淋巴细胞的比率;采用酶联免疫吸附技术(ELISA)夹心法检测研究对象血清中IL-2、IL-4及IFN-γ水平的变化。结果:与对照组比较,急性乙型肝炎患者外周血CD3+、CD4+T淋巴细胞、血清IL-2、IFN-γ水平明显升高(P<0.05);重型乙型肝炎患者CD3+、CD8+T淋巴细胞数量下降(P<0.05),IFN-γ水平升高(P<0.05);慢性乙型肝炎患者的IL-4和IFN-γ水平明显升高(P<0.05)。结论:Th1细胞因子在乙型肝炎病毒(HBV)感染的疾病发展中可能起一定的作用,HBV感染慢性化可能与Th2细胞因子增高有关。

Objective To study the T lymphoctye subsets and Th1/Th2 type cytokines levels in peripheral blood of patients with hepatitis B infection at different stages and healthy people and its clinical significance and to provide experimental basis for diagnosis and therapy of the diseases.Methods The percentages of CD3＋,CD4＋ and CD8＋ T lymphocytes among total lymphocytes in the peripheral blood of patients with HBV infection at different stages including 14 cases of moderate chronic hepatitis B（CHB）,7 cases of severe CHB,8 cases of severe hepatitis B and 5 cases of acute hepatitis B and 20 healthy controls were detected by flow cytometry.The levels of IL-2,IL-4 and IFN-γ in serum were detected by ELISA method.Results Compared with control group,the levels of CD3＋,CD4＋T lymphocytes and the levels of IL-2 and IFN-γ in the patients in acute hepatitis B group were increased significantly（P〈0.05）;the levels of CD3＋ and CD8＋T lymphocytes and the level of IFN-γ in patients in severe hepatitis B group were higher（P〈0.05）;the levels of IL-4 and IFN-γ in the patients in moderate and severe CHB groups were increased（P〈0.05）.Conclusion Th1 cytokines might play a role in the development of hepatitis B and the increasing of Th2 cytokine may be related to the chronic process in the patients with HBV infection.