绿茶提取物表没食子儿茶素没食子酸酯对人肝癌细胞株HepG2裸鼠移植瘤新生血管生成的影响

The Effect of Green Tea Extractive Epigallocatechin Gallate on the Angiogenesis of HepG2 Transplanted Tumor in Nude Mice

目的通过复制人肝癌细胞株HepG2裸鼠皮下移植瘤模型,观察绿茶提取物表没食子儿茶素没食子酸酯(EGCG)干预对HepG2移植瘤新生血管生成的影响。方法瘤体接种复制HepG2移植瘤模型,荷瘤裸鼠20只随机分组,实验组给予EGCG溶液每日20 mg/(kg.只),腹腔注射3周,对照组给予等量灭菌注射用水3周,末次用药24 h,后处死裸鼠,剥离移植瘤。常规病理切片观察移植瘤组织结构;逆转录-聚合酶链式反应和免疫组织化学法检测移植瘤缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)mRNA及蛋白表达,并通过检测CD34表达计数瘤组织微血管密度(MVD)。结果组织病理学观察实验组移植瘤见大量坏死区,瘤体内血管数量明显少于对照组;实验组HIF-1α、VEGF mRNA及蛋白表达水平比对照组均明显下调(P<0.05),实验组MVD比对照组明显下降(P<0.05)。结论 EGCG可抑制荷瘤裸鼠HepG2移植瘤新生血管生成。

Objective To investigate the effect of epigallocatechin gallate （EGCG） on the angiogenesis of human hepatocellular carcinoma cell strain HepG2 transplanted tumors in nude mice. Methods BALB/C （n/n） nude mice were used to replicate HepG2 transplanted tumor model, and they were randomly divided into experimental and control groups. Each nude mouse of the experimental group was treated with EGCG by intraperitoneal injection. After 3 weeks, nude mice were humanely killed, and the histological structure of transplanted tumors was observed under light microscope. The mRNA and protein expressions of hypoxia-inducible factor-lα （HIF-lα） and vascular endothelial growth factor （VEGF） in transplanted tumors were analyzed through reverse transcription-polymerase chain reaction and immunohistochemistry, and the microvessel density （MVD） was marked by CD34. Results A number of necrosis appeared and tumor vessel was scarce in the experimental group. The mRNA and protein expressions of HIF-lα and VEGF in the experimental group were significantly down-regulated compared with the control group （P 〈 0.05）, and the MVD in the experimental group was significantly lower than the control group （P 〈 0.05）. Conclusion The green tea extractive EGCG may inhibit the angiogenesis of HepG2 transplanted tumors in nude mice.