F型肉毒梭菌培养液可滤性实验的初步研究

Preliminary study on filterability for culture of Clostridium botulinum type F

目的通过探索F型肉毒梭菌培养液可滤性实验,为现有生产工艺提供科学的优化方案。方法分别采用不同型号的过滤器,通过不同的过滤组合对F型肉毒梭菌培养液进行澄清过滤。结果采用K700P过滤器(过滤精度6.0～15.0μm)对F型肉毒梭菌培养液进行一级澄清过滤,采用50P过滤器(过滤精度0.5～0.85μm)对料液进行二级澄清过滤,对F型肉毒梭菌培养液具有良好的澄清效果。结论可滤性实验的探索对于F型肉毒梭菌培养液批生产量的扩大具有切实可行的指导意义。

Objective The preliminary study was carried out in filterability for the culture of Clostridium botulinum type F toxin in order to provide an optimal and practical plan for the current production technology. Methods Different filter groups were selected and used in the process of clarification for culture and related parameters were compared. Results It was K700P （6.0 - 15.0μm） in the first clarification and 50 P （0.5 -0.85 μm） in the second clarification that were se- lected as the best group in the test, which showed an excellent result for the culture clarification of type F toxin. Conclu- sions The investigation is a practical principle for a scale up of lot production amount in culture of Clostridium botulinum type F toxin.