川芎高频再生体系的建立

Establishment and Optimization of High-frequency Shoot Regeneration System of Ligusticum chuanxiong Hort.

为建立川芎(Ligusticum chuanxiong Hort.)高频再生体系,优化了诱导和分化培养基及培养条件。以叶柄为外植体,以MS为基本培养基,KT 2.0 mg/L+IAA 0.5 mg/L的激素组合对不定芽分化最有利。在此基础上,针对外植体来源、培养条件和愈伤组织继代时间3个因素进行优化。结果表明:采用川芎无菌苗叶柄作为外植体,黑暗条件下诱导出愈伤组织,再在光照下继代培养15 d后转入分化培养基中对不定芽诱导最为有利,分化率为44.4%。分化后得到的不定芽在含NAA 0.5 mg/L和IBA 0.5 mg/L的1/2MS培养基上生根率达90%,移栽存活率为95%。

We explored optimized induction and differentiation medium and culture conditions to establish a protocol of rapid clonal propagation of Ligusticum chuanxiong Hort.The petioles were the best explant and Murashige and Skoog（MS） medium containing 2.0 mg/L KT and 0.5 mg/L IAA was suitable for high-frequency shoot regeneration.The results suggested that callus were induced from petioles of aseptic seedlings in dark conditions.And then these callus were subcultured for 15 days in light conditions,about 44.4% frequency of shoot regeneration was induced.The rooting percentage of the regenerated shoots reached 90% on 1/2 MS supplemented with 0.5 mg/L NAA and 0.5 mg/L IBA.Approximately 95% rooted shoots survived in a greenhouse.