仿生溶液法检测材料生物活性准确性的研究

Simulated body fluid related to implant bioactivity: An in vitro study on dental implants

目的:探讨仿生溶液(SBF)检测种植体生物活性的准确性。方法:采用宏观形貌一致的两种商用种植体Osseotite和NanoTiteTM分别体外浸泡于仿生溶液及DMEM+10%胎牛血清培养基中14天,应用X-射线光电子能谱仪(XPS)、扫描电镜(SEM)检测其表面磷灰石形成情况。结果:SBF中的两种样本表面无明显晶体形成;DMEM培养基中Osseotite表面无明显晶体形成,NanoTiteTM表面磷灰石晶体成球状簇集,并伴大量有机分子附着;XPS显示NanoTiteTM经DMEM浸泡后表面磷灰石晶体为碳酸羟基磷灰石。结论:SBF作为材料表面生物活性的检测模型,可导致假阴性结果的出现。有机大分子对生物矿化层的形成具有显著影响,以DMEM培养基作为体外生物活性研究模型的检测结果可信度更高。

Objective： To determine whether simulated body fluid （SBF） is suitable to estimate surface bioactivity of dental implants or not. Method： Two types of commercial dental implants （Osseotite and NanoTite^TM） with the same macrotopography were investigated on apatite formation in vitro by soaking separately with SBF and DMEM for 14 days. The results were studied by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS） and X-ray photoelectron spectroscopy （XPS）. Result： No apatite formation was detected on all samples after soaking in SBF. But globular, uniform apatite-like mineral deposits, accompany with organic molecular attachment was detected on the NanoTiteTM surface by soaking it in DMEM. Conclusion：SBF does not appear to be suitable to estimate surface bioactivity of a material, but DMEM shows potential for immersion test, as the influence of organic molecular on the apatite formation.