里氏木霉和黑曲霉降解香蕉秆产可发酵糖的研究

Study on conversion of banana stalk into sugar by Trichoderma reesei and Apsergillus niger

研究了里氏木霉和黑曲霉以香蕉秆作为碳源生产纤维素酶的培养特性。采取30℃培养木霉30 h后接种黑曲霉,32℃混合培养,得到FPA和β-Gluase活性互补的酶系组成:FPA为920.6 U/g,β-Gluase为864.2U/g。对发酵曲降解香蕉秆的研究结果表明:当木霉纯培养曲和黑曲霉纯培养曲以2∶1混合酶解时,最大酶解得率达到30.6%,酶解得率比木霉纯培养曲提高16.8%;优化条件下混合培养的酶解得率为31.5%,达到最大酶解得率所需时间比木霉纯培养曲缩短4 h,酶解得率提高20.2%。混合培养不仅优化了纤维素酶系组成,提高了糖化效率,而且可大大简化生产全酶系纤维素酶的工艺。

The characters of cellulase production by Trichoderma reesei and Apsergillus niger using banana stalk as the substrate was studied. The activity complementary emzye system was obtained when Trichoderma reesei was cultivated 30 h below 30 ℃, then mixed culture of Trichoderma reesei and Apsergillus niger at 32 ℃. The activity of FPA and β-Gluase reached 920.6 U/g and 864.2 U/g,respectively. The result of saccharification of banana stalk by inulinase showed： the highest conversion coefficient of 30.6% was obtained when the saccharification was carried out at the ratio of two strains inulinase was 2：1, and conversion coefficient was enhanced 16.8% than pure culture of Trichoderma reesei; The conversion coefficient by mixed culture was 31.5% ,4 h was shortened and conversion coefficient was enhanced 20.2% under the optimal conditions. It indicated that mixed culture not only optimize the composition of celtulase, enhance the saceharification efficiency, but also simplify the production process of the cellulose greatly.