膜技术纯化褐藻胶的工艺研究

Study on Purification of Algin by Membrane Technology

以海带酸性提取液为原料,采用膜技术除杂纯化褐藻胶。比较了微滤膜和超滤膜的纯化效果,并探讨了淀粉酶酶解提取液对膜处理效果的影响。结果表明,与微滤膜相比,超滤膜纯化褐藻胶的收率更高;优化的诺维信480L型中温淀粉酶酶解条件为:pH值6.0,酶加量0.3‰,酶解温度50℃,酶解时间2h;提取液酶解后,微滤膜和超滤膜的膜通量分别提高了34.3%和27.3%,但对褐藻胶的质量指标影响较小。最终确定的优化方案为:先将提取液中的褐藻淀粉酶解,再采用超滤膜除杂,所得褐藻胶浓缩液质量接近国标规格,褐藻胶损失很少。

With acid extract of Laminaria japonica Aresch as raw material, algin was purified through re- moving impurities by membrane technology. The purification efficiency of microfiltration and ultrafiltration were compared and the influences of enzymolysis of the acid extract by amylase on the membrane treatment were investigated. The results showed that, ultrafiltration had higher yield than microfiltration. The optimum enzymolysis conditions were obtained as follows.pH value of 6.0,enzyme dosage of 0. 3%0, enzymolysis temper- ature of 50 ℃,enzymolysis time of 2 h. Enzymolyzed by amylase Novozyme 480L,the membrane flux of micro- filtration and ultrafiltration were enhanced by 34.30% and 27.30%, respectively, but the quality indexes of algin changed little. In conclusion,the optimized treatment mode was firstly enzymolyzing the extract with amylase, and then removing impurities with ultrafiltration membrane, by which quality of the condensation liquor ob- tained nearly met the national standard sepcification and the algin loss was the least.