卵巢肿瘤p16蛋白的表达及意义

Expression of p16 in Ovarian Tumors and Its Clinical Significance

目的 :研究 p16在卵巢肿瘤中的表达及意义。 方法 :应用免疫组化ABC法检测 133例卵巢肿瘤和 12例正常卵巢组织 p16的表达。 结果 :卵巢肿瘤与正常卵巢组织 p16表达有显著性差异。正常卵巢没有 p16表达的缺失 ,良性、交界性、恶性组p16表达明显下降 (P <0 .0 5 )。上皮性组晚期、残存癌灶≥ 2cm者 p16表达明显低于早期、残存癌灶 <2cm者 (P <0 .0 5 ) ;p16表达与组织类型、组织分化、淋巴结转移无关 (P >0 .0 5 )。生存分析表明p16尚不能作为卵巢癌的独立预后因素。结论 :p16蛋白在卵巢肿瘤中存在不同程度的缺失 ,与卵巢癌的发生、发展有一定关系。p16能否作为卵巢癌的预后指标尚需进一步研究。

Objective: To study the expression of p16 in ovarian tumors and its clinical significance. Methods: The expression of p16 was studied in 133 cases of ovarian tumor (OT) and 12 normal ovarian tissues (NOT) by immunohistochemical technique. Results: There was significant difference of p16 protein expression between OT and NOT ( P <0.001). Loss of p16 protein was not detected in NOT, but detected in benign ovarian tumor, borderline ovarian tumor and malignant ovarian tumor with a descending tendency ( P <0.05). No difference of p16 protein expression was found between epithelial ovarian tumor (EOT) and non-epithelial tumor ( P <0.05). In EOT group, the expression of p16 protein in specimens from advanced stage or large residual cancer was significantly lower than that of early stage or small residual cancer 2cm ( P <0.05) . No relationship between the expression of p16 and histologic type, differentiation or lymph node metastasis of the tumor was found ( P <0.05). Survival analysis revealed that p16 expression could not be a prognostic factor of ovarian tumor. Conclusion: Loss of p16 protein is observed in ovarian tumors and it is associated with tumorigenesis and development of the tumor. Therefore, lose of p16 protein may indicate the progression of ovarian cancer.