CD44剪接变异体在乳腺癌中的表达

The expression of CD44 splice variants in breast cancer

目的观察乳腺癌细胞系及原发性乳腺癌组织中CD44剪接变异体种类及其表达情况。方法 RT-PCR法检测乳腺癌细胞系MDA-MB-231、MDA-MB-435和20例原发性乳腺癌组织中CD44变异体表达情况,分析其与临床病理参数之间的关系。结果 RT-PCR分析显示,在已知的8种剪接变异体、乳腺癌细胞系MDA-MB-231、MDA-MB-435和所检测的全部乳腺癌组织标本中,检测到CD44剪接变异体1、2、3、4、5、6、8,其中CD44剪接变异体4、5表达水平较高。CD44剪接变异体与临床病理参数分析显示CD44剪接变异体与患者年龄、TNM分期、淋巴结转移、ER、PR表达无关。CD44剪接变异体1和CD44剪接变异体2 mRNA的高表达与较小的肿瘤直径有关;CD44剪接变异体4的mRNA高表达与组织学高级别有关;CD44剪接变异体2、6的mRNA高表达与Her-2低表达相关;CD44剪接变异体5的mRNA低表达和Her-2高表达相关。结论 MDA-MB-231、MDA-MB-435及所检测的乳腺癌组织标本中,CD44剪接变异体为异质性表达,以标准型CD44表达水平最高。

Purpose To investigate the type and expression of CD44 splicing variants in breast cancer cell lines and breast cancers. Methods RT-PCR were used to examine the expression levels of CD44 splicing variants in the breast cancer cell lines MDA-MB-231, MDA-MB-435 and 20 cases of breast cancer tissue samples. The association between expression of CD44 splicing variants with clinieo- pathologic parameters were assessed. Results RT-PCR revealed that the CD44 splicing variants 1,2, 3, 4, 5, 6, 8 were detected in the breast cancer cell lines MDA-MB-231, MDA-MB-435 and breast cancer tissue samples in the known eight CD44 splicing variants, the expression of CD44 splicing variants4, 5 were higher in all detected CIM4 splicing variants. CD44 splicing variants and clinicopath- ological parameters analysis showed that the CD44 splicing variants were unrelated to the patients age, TNM staging, lymph node me- tastasis, ER and PR expression. A high expression of CI）44 splicing variants 1 and 2 mRNA were correlated to smaller tumor diameter, high expression of CD44 splicing variants 4 mRNA was correlated to higher histology grade. Negative Her-2 status were correlated to ex- pression of CD44 splicing variants 2, 6, but strong Her-2 status was correlated to expression of CD44 splicing variants 5 mRNA. Con- clusion The breast cancer cell lines MDA-MB-231, MDA-MB-435 and breast cancer tissue samples showed a heterogeneous expres- sion pattern of CD44 splicing variants. The standard CD44 expressed highest in both MDA-MB-231, MDA-MB-435 and breast cancer tissue samples.