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胃腺癌中p57^kip2和LIMK-1的表达及意义 被引量：3

Significanca of the expression of p57^(kip2),LIMK-1 in gastric cancer
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摘要 目的探讨p57kip2mRNA、LIMK-1mRNA及其蛋白在胃腺癌组织中的表达,了解其与肿瘤组织分化程度及临床病理特征的关系。方法采用显色原位杂交(chromogenic in situ hybridizations,CISH)技术和免疫组化EnVision法检测40例胃腺癌及癌旁正常胃黏膜组织中p57kip2mRNA、LIMK-1mRNA及其蛋白的表达。结果 (1)正常胃黏膜组织中p57kip2高表达,在胃腺癌组织中低表达,差异具有统计学意义(P<0.05);(2)p57kip2的表达与胃腺癌组织分化程度呈负相关(P<0.05);(3)p57kip2、LIMK-1蛋白表达与胃腺癌组织中微脉管计数微淋巴管密度(lymphaticmicrovessel density,LVD)、微血管密度(microvesseldensi-ty,MVD)呈正相关(P<0.05)。结论胃腺癌存在p57kip2基因组印记缺失,致p57kip2mRNA及其蛋白表达减少或缺失,并与胃腺癌组织分化程度有关,LIMK-1蛋白高表达与肿瘤的浸润转移及微脉管的生成有关,检测胃癌活检标本中LIMK-1蛋白的表达,对预测胃腺癌预后及浸润转移有辅助诊断意义。 Purpose To explore the expression of p57kip2, LIMK-1 in gastric adenocarcinoma tissues, and to analyze their correlation be- tween the expression of p57^kip2, LIMK-1 and tumor differentiation and biological behavior. Methods The expressions ofp57^kip2and LIMK-1 protein and their mRNA in 40 cases of gastric adenocarcinoma were detected by EnVision immunohistochemistry and digoxigenin labeled oligonucleotide probe in situ hybridization, respectively. Results （ 1 ） Expression of p57^kip2in normal gastric mucosa was higher than that in the cancerous tissues, the difference was statistically significant （P 〈 0. 05）. （2） The relationship between the expression of p57^kip2 and histological grade were negatively correlated in gastric cancer （P 〈 0. 05 ）. （3） Expression of p57^kip2, LIMK-1 was positively correlated with LVD and MVD in gastric adenoearcinoma （ P 〈 0. 05 ）. Conclusion The low expression of p57^kip2 and the high expression of LIMK-1 may be important biological markers of malignant transformation of gastric mucosa and the lymph node metastasis of gastric ade- nocarcinoma, and it is important in predicting prognosis, invasion and metastasis of the gastric adenocareinoma.
作者 贺晓燕 于燕妮 王龙
机构地区 贵州省肿瘤医院病理科 贵阳医学院病理教研室
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2012年第7期756-760,共5页 Chinese Journal of Clinical and Experimental Pathology
关键词 胃肿瘤 腺癌 P57KIP2 LIMK-1 肿瘤转移 免疫组织化学 原位杂交 gastric neoplasms adenocarcinoma p57kip2 LIMK-1 tumor metastasis immunohistochemistry ISH
分类号 R735.2 [医药卫生—肿瘤]
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参考文献12

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二级参考文献31

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共引文献1

同被引文献35

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二级引证文献6

临床与实验病理学杂志
2012年 第7期

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