期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

53BP1在宫颈鳞状细胞癌及其癌前病变中的表达 被引量：2

Expression of 53BP1 in squamous cell carcinoma and precancerous lesions of the cervix
下载PDF
导出
摘要 目的探讨53BP1在宫颈鳞状细胞癌及其癌前病变中的表达,阐明53BP1在宫颈鳞状细胞癌发生中的作用。方法应用免疫组化EnVision法检测10例正常宫颈组织、20例宫颈鳞状上皮内瘤变1级(cervical intraepithelial neoplasm 1,CIN1)、20例CIN2、20例CIN3和20例宫颈鳞状细胞癌中53BP1的表达。结果正常宫颈组织中仅有2例53BP1呈局灶性表达,阳性率为20%,而53BP1蛋白在CIN1～3和宫颈癌组织中阳性率均为100%,差异有统计学意义(P<0.01)。53BP1在正常宫颈组织和CIN1中弥漫阳性率均为0,而在CIN2、CIN3和宫颈癌组织中弥漫阳性率分别为55%、70%和100%,53BP1在CIN2、CIN3和宫颈癌组织中的弥漫阳性表达明显高于正常宫颈组织和CIN1(P<0.01)。53BP1蛋白弥漫阳性表达在CIN2与CIN3之间差异无显著性(P=0.327)。宫颈癌中53BP1蛋白弥漫阳性表达明显高于CIN3和CIN2,差异有统计学意义(P=0.020;P=0.001)。结论 53BP1表达水平可作为基因组不稳定性的标记物,基因组不稳定性在宫颈癌发生过程中可能是一早期事件。 Purpose To investigate the expression of 53BP1 in squamous cell carcinoma and precancerous lesions of the cervix, and to expolore the role of 53BP1 in the carcinogenesis of cervical cancer. Methods Immunohistochemieal EnVision was used to detect the expression of 53BP1 in 10 cases of normal cervix tissue, 20 cases of cervical intraepithelial neoplasm 1 （CIN1）, 20 cases of CIN2, 20 cases of CIN3, and 20 cases of squamous cell carcinoma. Results Focal positive expression of 53 BP1 was seen in 2 cases of normal cervical tissue and the positive rate of 53BP1 in the normal cervix was 20%. The positive rates of 53BP1 in CIN1, CIN2, CIN3 and cancer were all 100%. There was significant differences between normal tissue and CIN1, CIN2, CIN3 and cancer（ P 〈 0. 01 ）. The diffuse positive rates of 53BP1 in the normal cervix and CIN1 were 0, but the diffuse positive rates of 53BP1 in the CIN2, CIN3 and cancer were 55% , 70% and 100%. The diffuse positive rates of 53BP1 in the CIN2, CIN3 and cancer were higher than that in normal tissue and CIN1 （P 〈0. 01 ）. There was no difference of 53BP1 expression between CIN2 and CIN3 （P =0. 327）. The diffuse positive expression of 53BP1 in the squamous cell carcinoma was higher than that in CIN3 and CIN2 （ P = 0. 020, P = 0. 001 ）. Conclusions Expression of 53BP1 may be a marker for genomic instability and genomic instability may be a early event in the carcinogenesis of cervi- cal cancer.
作者 周熹 何家玉
机构地区 芜湖市妇幼保健院妇产科 芜湖广济医院病理科
出处 《临床与实验病理学杂志》 CAS CSCD 北大核心 2012年第7期772-774,共3页 Chinese Journal of Clinical and Experimental Pathology
关键词 子宫颈肿瘤 53BP1 基因组不稳定性 肿瘤发生 cervical neoplasms 53BP1 genomic instability carcinogenesis
分类号 R737.3 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1唐卓葳,吴诚义.53BP1在乳腺癌中的表达及其意义[J].中国普通外科杂志,2009,18(5):518-521. 被引量：2
  • 2Lengauer C, Kinzler K W, Vogelstein B. Genetic instabilities in human cancers [ J ]. Nature, 1998,396 (6712 ) :643 - 9.
  • 3Shiloh Y, Kastan M B. ATM: genome stability, neuronal develop- ment, and cancer cross paths[J]. Adv Cancer Res,2001,83 :209 -54.
  • 4Matsuda K, Miura S, Kurashige T, et al. Significance of p53- binding protein 1 nuclear foci in uterine cervical lesions: endoge- nous DNA double strand breaks and genomic instability during car- cinogenesis [ J ]. Histopathology, 2011,59 ( 3 ) : 441 - 51.
  • 5Iwabuchi K, Bartel P L, Li B, et al. Two cellular proteins that bind to wild-type but not mutant p53 [ J 1. Proc Natl Acad Sci USA,1994,91 (13) :6098 - 102.
  • 6Iwabuchi K, Li B, Massa H F, et al. Stimulation of p53-mediated transcriptional activation by the p53,binding proteins, 53BP1 and 53BP2[J]. J Biol Chem,1998,273(40) :26061 -8.
  • 7Ward I M, Minn K, Jorda K G, et al. Accumulation of checkpoint protein 53BP1 at DNA breaks involves its binding to phosphoryla- ted histone H2AX [ J ]. J Biol Chem,2003,278 (22) : 19579 - 82.
  • 8Gorgoulis V G, Vassiliou L V, Karakaidos P, et al. Activation of the DNA damage checkpoint and genomic instability in human pre- cancerous lesions [J]. Nature, 2005,434 ( 7035 ) :907 - 13.
  • 9杜然,张惠箴,陈杰,蒋智铭.53BP1蛋白表达缺失与前列腺腺癌临床病理的关系[J].临床与实验病理学杂志,2011,27(1):44-47. 被引量：8

二级参考文献34

  • 1李科,杨红,董丹丹.抗体鸡尾酒p504s/p63标记在前列腺癌诊断中的应用[J].临床与实验病理学杂志,2006,22(3):372-372. 被引量：2
  • 2Naruke Y, Nakashima M, Suzuki K, et al. Alteration of p53-binding protein 1 expression during skin carcinogenesis: association with genomic instability [J]. Cancer Sci, 2008, 99(5) :946 -951.
  • 3Bartkova J, Horejsi Z, Sehested M, et al. DNA damage response mediators MDC 1 and 53 BP 1 : constitutive activation and aberrant loss in breast and lung cancer, but not in testicular germ cell tumours [ J ] . Oncogene, 2007 , 26 ( 53 ) : 7414 -7422.
  • 4Kobayashi J, Iwabuchi K, Miyagawa K, et al. Current topics in DNA double-strand break repair [ J ]. J Radiat Res, 2008,49(2) :93 - 103.
  • 5Ward IM, Minn K, Jorda KG, et al. Accumulation of checkpoint protein 53 BP 1 at DNA breaks involves its binding to phosphorylated histone H2AX [ J ]. J Biol Chem, 2003, 278(22) :19579-19582
  • 6Motoyama N, Naka K. DNA damage tumor suppressor genes and genomic instability [ J ]. Curr Opin Genet Dev, 2004, 14(1) :11 -16.
  • 7Nakashima M, Suzuki K, Meirmanov S, et al. Foci formation of P5 3-binding protein 1 in thyroid tumors: activation of genomic instability during thyroid carcinogenesis [ J ]. Int J Cancer, 2008 , 122 (5) : 1082 - 1088.
  • 8Gorgoulis VG, Vassiliou LV, Karakaidos P, et al. Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions [ J ]. Nature, 2005, 434 (7035) :907 -913.
  • 9Zhu C, Mills KD, Ferguson DO, et al. Unrepaired DNA breaks in p53-deficient cells lead to oncogenic gene amplification subsequent to translocations [ J ]. Cell, 2002, 109 (7) :811 -821.
  • 10Rauch T, Zhong X, Pfeifer GP, et al. 53 BP1 is a positive regulator of the BRCA1 promoter[ J]. Cell Cycle. 2005,4 (8) :1078-1083.

共引文献7

同被引文献21

  • 1Guerra B, Iwabuchi K, Issinger OG. Protein kinase CK2 is required for the recruitment of 53BPI to sites of DNA double - strand break induced by radiomimetic drugs[J]. Cancer Lett, 2014, 345 (1) : 115 -123.
  • 2Ward 1M, Difilippantonio S, Minn K, et al, 53BPI cooperates with p53 and functions as a haploin sufficient tumor suppressor in mice[J] . Mol Cell Bioi, 2005, 25 (22) : 10079 - 10086.
  • 3Gupta A, Hunt CR. Role of 53 BPI in the regulation of DNA doublestrand break repair pathway choice[J]. Radiat Res, 2014, 181 (1) : 1-8.
  • 4Carr SM, Munro S, Zalmas LP, et al. Lysine methylation - dependent binding of 53BPI to the pRb tumor suppressor[J]. Proc Natl Acad Sci USA, 2014,111(31): 11341-1146.
  • 5Hong S, Li X, Zhao Y, et al. 53 BPI suppresses tumor growth and promotes susceptibility to apoptosis of ovarian cancer cells through modulation of the Akt pathway[J]. Oncology Reports, 2012, 27 (4) :1251 -1257.
  • 6ChapmanJR, Barral P, VannierJB, et al. RIFI is essential for 53 BPI - dependent nonhomologous endJoining and suppression of DNA double - strand break resection[J]. Mol Cell, 2013, 49 (5) : 858 - 871.
  • 7Rauch T, Zhong X, Pfeifer GP, et af. 53 BPI is a positive regulator of the BRCAI promoter[J]. Cell Cycle, 2005, 4 (8) : 1078 - 1083.
  • 8Pennington KP, Wickramanayake A, Norquist BM, et al. 53BPI expression in sporadic and inherited ovarian carcinoma: relationship to genetic status and clinical outcomes[J]. Gynecol Oncol, 2013, 128 (3) :493 - 499.
  • 9Rapakko K, Heikkinen K, Karppinen SM,et al, Germline alterations in the 53 BPI gene in breast and ovarian cancer families[J]. Cancer Lett, 2007,245(1 -2) :337 -340.
  • 10Ahmed N, Abubaker K, FindlayJ. Epithelial mesenchymal transition and cancer stem cell - like phenotypes facilitate chemoresistance in recurrent ovarian cancer[J]. Current cancer drug targets, 2010, 10 (3) :268 -278.

引证文献2

二级引证文献2

临床与实验病理学杂志
2012年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部