DICER基因多态性与食管癌淋巴结转移危险因素的关系

Association of Variant Allele of DICER and Decreased Risk of Esophageal Cancer Lymph Node Metastasis

目的探讨中国汉族人群中DICER基因rs3742330多态性与食管癌淋巴结转移危险因素的关系。方法采用以医院为基础的病例-病例研究方法,采用基质辅助激光解析电离飞行时间质谱(MAL-DI-TOF MS)技术分析85例有淋巴结转移的食管癌和270例无淋巴结转移的食管癌DICER基因rs3742330基因多态性,计算各种基因型与食管癌淋巴结转移的发生风险及其95%可信区间。结果 DICER rs3742330 A>G多态三种基因型AA、AG、GG在食管癌淋巴结转移组的频率分别为31.76%、61.18%、7.06%,在无淋巴结转移组的频率分别为34.44%、48.15%、17.41%,与携带DICERrs3742330 AA基因型的个体相比较,DICER rs3742330 GG基因型显著减少食管癌淋巴结转移发生的危险。结论 DICER rs3742330 A>G基因多态性可能是食管癌淋巴结转移的保护性因素。

Objective To investigate the relationship between DICER polymorphism and the susceptibility to lymph node metastasis in esophageal cancer in Chinese Han population.Methods Genotypes were determined by matrix-assisted laser desorption /ionization time-of-flight mass spectrometry（MALDI-TOF MS） method in 85 lymph node metastasis positive patients and 270 lymph node metastasis negative patients.Results The DICER rs3742330 A〉G genotype frequencies were 31.76%（AA）,61.18%（AG）,7.06%（GG） in the lymph node positive group and 34.44%（AA）,48.15%（AG）,17.41%（GG） in the lymph node negative group,respectively.Logistic regression analyses revealed that the risk associated with DICER rs3742330 GG genotype was 0.36（95%CI=0.15~0.88） which was much lower than DICER rs3742330 AA＋AG genotype.Conclusion DICER rs3742330 A〉G polymorphism may be a protective factor of lymph node metastasis.