摘要
利用在醋酸 醋酸钠缓冲溶液 (pH6.0 )中Cu2 +与铱来铬青蓝R形成紫红色络合物的显色反应 ,建立了一种新的测定痕量铜的分光光度法。该络合物的最大吸收波长为 565nm ,摩尔吸光系数为 8.2 5× 1 0 5L·mol- 1 ·cm- 1 ,Cu2 +在 0～ 0 .48μg·ml- 1 范围内服从比尔定律。该法灵敏度高 ,操作简便 ,用于正常人及类风湿性关节炎患者头发中的痕量铜分析 ,结果发现 。
A new color reaction of copper (II) with solochrome cyanine R was studied by spectrophotometry. The results were that in the pH6 of HAc NaAc buffer solution solochrome cyanine R reacted with copper (II) to form a violet complex; the maximum absorption of the complex was at 565 nm and its apparent molar absorption coefficient was 8.25×10 5 L·mol -1 ·cm -1 . Beer's Law was obeyed in the range of 0～0.48 μg·ml -1 . This method was used to determine copper levels in the hair samples of 5 normal persons and 5 rheumatoid arthritis patients. The results showed that the hair copper level of arthritis patients was significantly higher than that of normal persons (P<0.01). [
出处
《湖南医科大学学报》
CSCD
2000年第2期117-118,共2页
Bulletin of Hunan Medical University
基金
湖南省卫生厅资助课题 !( 983 6)
