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硬组织替代材料对成骨细胞内游离钙及钙调素水平的影响 被引量:2

In Vitro Studies about the Effects of Implant Materials on Calmodulin,Introcellular Calcium of Rat Osteoblasts
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摘要 目的 :研究羟基聚磷酸钙钠 (HPA)、羟基磷灰石 (HA)、生物玻璃陶瓷 (BGC)和纯钛 (Ti) 4种硬组织替代材料对成骨细胞的细胞内游离钙 ([Ca2 + ]i)浓度、钙调素 (CaM)含量以及细胞膜ATP酶活性的影响 ,探讨这 4种材料的生物相容性。方法 :选用SD乳鼠颅顶骨成骨细胞进行体外培养 ,把HPA、HA、BGC和Ti粉分别制成材料浸提液 ,将培养成活的成骨细胞接种于材料浸提液中培养 ,3d后测定成骨细胞内 [Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性。结果 :4种材料对细胞内游离钙浓度无不良影响 ,钛对钙调素含量有明显抑制作用 (P <0 0 5 ) ,HPA、HA、BGC 3种材料对细胞膜Ca2 + ATP酶活性有增强作用 (P <0 0 5 ) ,HPA、HA 2种材料对Na+ 、K+ ATP酶活性有增强作用 (P <0 0 5 )。结论 :[Ca2 + ]i 浓度、CaM含量及细胞膜ATP酶活性能够反映不同硬组织替代材料的生物相容性 ,可以为建立和完善评价硬组织替代材料生物相容性的方法和指标提供依据。 Objective: ①To evaluate the effects of the four implant materials, hydroxyl poly calcium sodium phosphate (HPA), hydroxyapatite (HA), bioglass ceramics (BGC) and titanium (Ti) on the calmodulin (CaM) content of osteoblasts, the concentration of intracellular free calcium and the ATPases activity of cell membrane of rat osteoblasts cultured in vitro; ②To reveal the biological compatibility of the four implant materials and the index to estimate the biological compatibility condition. Methods: The powders of HPA, HA, BGC and Ti were sterilized and made into extracted solutions with 199 culture medium. The isolated osteoblasts, which were taken from three day old sparague Dawley rats′ calvaria, were incubated in 199 culture medium also. Then the osteoblasts were mixed and incubated into the four kinds of extracted solutions of the implant materials. After three days of co incubation, the cells were taken out, and the cellular calmodulin content were measured with radiobiological analysis. The concentration of intracellular free calcium were under fluorescent analysis, and the ATPases activities of cell membrane were estimated with colorimetric analysis. Results: ①Calmodulin content of the osteoblasts co incubated with all the implant materials decreased, and moreove, only that of the Ti group decreased more obviously than that of the Ti and HPA group (P<0.05). ②The concentration of intracellular free calcium of all the co incubated osteoblasts were as normal as that of the control group. ③Ca\+\{2+\} ATPases activities of osteoblasts co incubated with HPA, HA, BGC increased (P<0.05), and Na\++/K\++ ATPases activities of the cells co incubated with HPA, HA were strenghtened (P<0 05). Conclusion: The indexes of cellular calmodulin content,concentration\;of intracellular free calcium and ATPases activity of cell membrane can reflect the biological compatibility of implant materials, and they may be used as indexes of estimating biological compatibility condition.
出处 《华西口腔医学杂志》 CAS CSCD 北大核心 2000年第2期119-122,共4页 West China Journal of Stomatology
基金 国家自然科学基金!(编号 3 93 3 0 0 90 )
关键词 硬组织替代材料 成骨细胞 细胞内游离钙 钙调素 implant material osteoblast calmodulin intracellular free calcium ATPase
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