Anti-CCP和RF联合检测在诊断RA中的意义

Significance of detecting anti-cyclic citrullinated peptide and rheumatoid factor in the diagnosis of rheumatoid arthritis

目的分析比较抗环瓜氨酸肽抗体(Anti-CCP)和类风湿因子(RF)在类风湿关节炎(RA)中的灵敏度和特异性,探讨将两种指标联合检测在RA诊断中的价值。方法利用酶联免疫吸附试验和乳胶增强免疫透射比浊法分别测定76例RA患者,56例非RA患者血清中的Anti-CCP和RF。结果 Anti-CCP和RF在RA组的阳性率明显高于非RA组。特异度和灵敏度RF分别为76.8%、78.9%,Anti-CCP分别为96.4%、60.5%,将两者联合检测则分别为86.6%、73.1%。结论 Anti-CCP在诊断RA中具有较高的特异性和灵敏度,可作为诊断RA的新的血清学指标。将Anti-CCP和RF联合检测有助于RA的临床诊断。

Objective To analyse the specificity and sensitivity of anti-cyclic citrullinated peptide （Anti-CCP） and rheumatoid factor （RF） in the diagnosis of rheumatoid arthritis （RA） to investigate the value when the two antibodies were used together. Methods ELISA and Latex enhanced immune turbidimetric were used to detect serum levels of Anti-CCP and RF in 76 patients with RA and 56 nonRA patients. Results The positive rate of Anti-CCP and RF of patients with RA was significantly higher than that of non-RA patients. The diagnostic specificity of RF for RA was 76.8% and the sensitivity was 78.9%, whereas the diagnostic specificity of Anti-CCP for RA was 96.4% and the sensitivity was 60.5%. The diagnostic specificity of the Anti-CCP and RF was 86.6% and the sensitivity was 73.1%. Conclusion The Anti-CCP might have high specificity and sensitivity, which might be used as the new serum sign for the diagnosis of RA. Combined with the detection of Anti-CCP and RF ,the sensitivity will be higher.