摘要
Objective:To investigate whether remifentanil induced cardioprotecting effect is associated with expression of toll-like receptor 4(TLR4),nuclear factor κB(NF-κB) and serum interleukin-6(IL-6).Methods:Fifty rabbits were randomly divided into 5 groups(n=10) according to the treatment:sham operation group(group A),ischemia-reperfusion group(group B),low-dose remifentanil group(group C),mediate-dose remifentanil group(group D),and high-dose remifentanil group(group E).Myocardial TLR4 mRNA levels,NF-κB protein expression and serum levels of IL-6 were observed in 120 min after reperfusion.Results:The myocardial expressions of TLR4 mRNA,NF-κB protein and IL-6 level in sera of groups B,C,D and E were elevated compared with group A.However,remifentanil significantly reduced the levels of TLR4 mRNA,NF-κB protein expression and serum IL-6 in groups C,D and E compared with group B.There were remarkable differences between the groups(P<0.01).Conclusion:Intravenous remifentanil has protective effect against rabbit myocardial ischemia/reperfusion injury.This effect may be associated with TLR4,NF-κB expressions on myocytes and serum level of IL-6 in a dose-dependent manner.
Objective: To investigate whether remifentanil induced cardioprotecting effect is associated with expression of toll-like receptor 4 (TLR4), nuclear factor rB (NF-r.B) and serum interleukin -6 (IL-6). Methods: Fifty rabbits were randomly divided into 5 groups (n=10) according to the treatment: sham operation group (group A), ischemla-reperfusion group (group B), low-dose remifentanil group (group C), mediate-dose remifentanil group (group D), and high-dose remlfentanil group (group E) Myocardial TLR4 mRNA levels, NF-r.B protein expression and serum levels of IL-6 were observed in 120 min after reperfusion. Results: The myocardial expressions of TLR4 mRNA, NF-rd3 protein and IL-6 level in sera of groups B, C, D and E were elevated compared with group A. However, remifentanil significantly reduced the levels of TLR4 mRNA, NF- r.B protein expression and serum IL-6 in groups C, D and E compared with group B. There were remarkable differences between the groups (P〈O.O1). Conclusion: Intravenous remifentanil has protective effect against rabbit myocardial ischemia/reperfusion injury. This effect may be associated with TLR4, NF-r.B expressions on myocytes and serum level of IL-6 in a dose-dependent manner
基金
Supported by Shaanxi Provincial Scientific and Technological Research Projects (2008K13-02)