氧化酚砷对血液肿瘤细胞系的体外效应

In vitro effects of phenylarsine oxide on hematopoietic malignant cell lines

目的：了解氧化酚砷（Ｐｈｅｎｙｌａｒｓｉｎｅ ｏｘｉｄｅ， ＰＡＯ）对血液肿瘤细胞系的影响。方法： ８种血液肿瘤系经０．０１μｍｏｌ／Ｌ、０.０５μｍｏｌ／Ｌ和０．１μｍｏｌ／Ｌ的ＰＡＯ处理达一定时间后，经台盼蓝排除法计数细胞活力和应用流式细胞仪检测细胞ＤＮＡ含量分布。结果和结论：在生长抑制和诱导凋亡方面，ＰＡＯ对血液肿瘤细胞的效应谱相对较广，０．０５μｍｏｌ／Ｌ和０．１μｍｏｌ／Ｌ的ＰＡＯ能抑制某些血液肿瘤细胞的生长，并诱导其凋亡。但是，不同来源的血液肿瘤细胞系对ＰＡＯ的敏感性不完全相同。

Objective: To investigate effects of phenylarsine oxide (PAO) on hematopoietic maligant cell lines. Methods: Eight cell lines from hematopoietic malignancies were treated with 0. 01 μmol/L, 0. 05 μmol/L and 0. 1μmol/ L of PAO for different days. Cell viability was assessed by trypanblau exclusion, and the distribution of cellular DNA contents was measured on flow cytometry. Results and Conclusion: PAO had a wider effect spectrum on hematopoietic malignant cell lines in terms of growth inhibition and apoptosis induction, and 0. 05 μmol/L and 0. 1μmol/L of PAO could inhibit growth of some hematopoietic malignant cells with or without apoptosis induction. However, cell lines from different origins had the different sensitivity to PAO.