RNasin对脑胶质瘤细胞生长的抑制作用

Suppression effect of ribonuclease inhibitor on the growth of glioma cells

目的：探讨核糖核酸酶抑制因子（ＲＮａｓｉｎ）对脑胶质瘤生长的抑制作用及可能机理。方法：从人胎盘组织中提取制备ＲＮａｓｉｎ，体内外观察其对人ＳＨＧ４４和大鼠Ｃ６胶质瘤细胞生长的抑制作用。结果：（１）体外不同浓度的ＲＮａｓｉｎ对培养的Ｃ６及ＳＨＧ４４胶质瘤细胞存活率无明显影响，与对照组比较无显著性差异（Ｐ＞０．０５）；（２）加入ＲＮａｓｉｎ后裸鼠体内Ｃ６胶质瘤平均重量为１．７２ ｇ±０．１８４ ｇ，平均大小（直径）为１２．８４ ｍｍ±１.１５ ｍｍ；ＳＨＧ４４胶质瘤平均重量为１．０１２ ｇ±０．１７４ ｇ，平均大小为１１．２０ ｍｍ±１．２１ｍｍ，均与对照组有显著性差异（Ｐ＜０．０５）。结论：ＲＮａｓｉｎ体外对培养的胶质瘤细胞生长无抑制作用，而体内对胶质瘤瘤体的生长有明显抑制作用。

Objective: To explore the role and mechanism of suppression of ribonuclease RNase inhibitor in the growth of glioma cells. Method: Ribonuclease inhibitor was extracted from human placenta, and the suppression of ribonuclease inhibitor on the growth of human glioma cell line SHG44 and rat glioma cell line C6 was observed in vitro and in vivo. Results: (1 ) Different concentrations of ribonuclease inhibitor had no prominent effect on the survival rates of two glioma cell lines in vitro. There was no remarkable difference between experimental and control groups (P >0.05). (2) the average weight and size of tumor in nude mice in treated and control groups were that: C6 glioma cell line 1. 172±0. 184 g vs 2. 876 g±3. 94 g and 12. 84±1.15 mm vs 21.72 mm±1. 60 mm; SHG44 glioma cell line 1. 012±0. 174 g vs 2. 834± 0. 321 g and 11. 20 ±1. 21mm vs 22.17±1.62 mm. There were evidences differences between experimental and control groups (P <0.01). Conclusion: Ribonuclease inhibitor has no inhibitory effect on the growth of cultured glioma cells in vitro, whereas it has significant inhibitor effect on the growth of glioma in vivo.