高效液相色谱法检测酸牛乳中纳他霉素含量的几种前处理方法比较

Comparison of pretreatment methods in determination of natamycin content in sour Milk by HPLC

在酸牛乳基质中分别添加0.64 mg/L、1.28 mg/L、3.2 mg/L、6.4 mg/L、12.8 mg/L5个浓度的纳他霉素标准品。分别用几种方法进行了酸牛乳样品前处理,高效液相色谱仪测定,外标法定量。比较了几种方法的回收率、检出限及通过滤膜难易程度。结果表明:方法的回收率为66%～103%,检出限为0.02 mg/kg～0.03 mg/kg。

Differentconcentrationsofnatamycinstandards （0.64rag/L, 1.28rag/L, 3.2mg/L, 6.4mg/L, 12.8mg/L） were added in sour milk. Several methods were used to the pretreatment of sour milk samples. They are assayed by HPLC and quantitative determined by external standard method. Recovery rate, detection limit, and the difficulty degree of filtration membrane were compared. Results showed that the recovery rate was between 66%-103% and the detection limit was 0.02 mg/ kg - 0.03 mg /kg.