摘要
目的 探讨线粒体 DNA与人精子活力间的关系。 方法 用长链 PCR技术 ,对 6 0例精子活力正常和 40例精子活力异常不育患者的精子线粒体 DNA(mt DNA)进行了多重缺失的分析。 结果 两组不育患者中共有 8例具有 m t DNA的多重缺失 (其中精子活力正常不育患者 6名 ,精子活力异常不育患者 2名 ) ,但缺失型 mt D-NA(S5除外 )在总 mt DNA中所占比例很小 (0 .16 %~ 1.85 % ) ,1例精子活力正常的不育患者 (S5 )具有 2 .6 kb的缺失型 mt DNA,且缺失型 mt DNA占总 m t DNA的 91.0 2 % ,其序列分析发现 mt DNA编码区域大部分缺失。 结论 精子活力与 mt DNA的多重缺失间无相关性。
Objective\ To analyze the correlation between mitochondrial DNA and human sperm mobility. Methods By using long polymerase chain reaction techniques, we studied multiple deletions of sperm mitochondrial DNA(mtDNA) in 100 infertile patients(60 with normal sperm mobility and 40 asthenospermia). Results Six of 60 patients with normal sperm mobility and two of 40 asthenospermia had multiple mtDNA deletions, but percentage of deletions in mtDNA(DmtDNA) in total mtDNA was very low(from 0 16% to 1 85%).A 2 6kb DmtDNA was found in one of infertile patients (No.S5) with normal sperm mobility, and percentage of 2 6kb DmtDNA in total mtDNA was 91 02%. Sequence analysis revealed that most of mtDNA sequence encoding RNA and polypeptide were deleted. Conclusion There is no relationship between multiple deletions in mtDNA and human sperm mobility.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第1期34-38,共5页
Acta Anatomica Sinica
关键词
线粒体
DNA
精子活力
长链
聚合酶链反应
Mitochondrial DNA
Sperm mobility
Long polymerase chain reaction