肿瘤组织中端粒酶活性的研究

THE STUDY OF TELOMERASE ACTIVITY IN TUMORS

目的 了解端粒酶活性高低与肿瘤恶性程度关系。 方法 用 TRAP- EL ISA和我们改进的 TRAP银染方法 ,对肿瘤组织端粒酶活性进行半定量检测。 结果 端粒酶阳性率分别为 :胶质瘤星形 级 40 % , 和 级 10 0 % ,肠癌 84.6 2 % ,食管癌 90 %。 结论 端粒酶活性与肿瘤恶性程度密切相关 ,是诊断恶性肿瘤、判断愈后的良好指标。

Objective\ To investigate the correlation between telomerase activity and pathologic grade of the tumors.Methods We detected telomerase activity in tumors by TRAP ELISA and TRAP Ag + stain methods.Results Telomerase positive rate is 40% for astrocytoma Ⅱ, 100% for astrocytoma Ⅲ and Ⅳ,84 62% for intestinal cancer, 90% for esophaeal carcinoma. Conclusion There is a significant correlation between telomerase activity and pathologic grade of the tumors.