四氧化三铁磁性纳米颗粒对人角质形成细胞超微结构的影响

Influence of ferroferric oxide magnetic nanoparticles on ultrastructure of human keratinocyte cells

目的研究四氧化三铁(磁性Fe3O4)纳米颗粒对人角质形成细胞(HaCaT)超微结构的影响。方法将不同浓度的Fe3O4磁性纳米颗粒悬液加入培养的HaCaT细胞中,在37℃、5%CO2的细胞培养箱中共同孵育4h,收集细胞进行常规透射电镜制样,在透射电镜下观察纳米Fe3O4颗粒进入细胞的方式及细胞超微结构的改变。结果 Fe3O4磁性纳米颗粒平均粒径为12nm。在不同浓度下Fe3O4磁性纳米颗粒均能以吞噬的方式进入细胞,进入细胞后再从吞噬泡中释出,主要对其附近的线粒体造成影响,线粒体出现肿胀,线粒体嵴溶解。结论 Fe3O4磁性纳米颗粒主要对HaCaT细胞中颗粒附近的线粒体结构有损伤,且存在浓度依赖性。

Objective To investigate the influence of Fe3 04 magnetic nanoparticles on human keratinocyte cells （HaCaT cells）. Methods HaCaT cells were incubated with different concentrations of Fe3O4magnetic nanoparticles for 4 h at 37℃ with 5 % CO2. Then transmission electron microscopy （TEM） was used to observe the way nanoparticles entering HaCaT cells and the ultrastructure of HaCaT cells. Results The mean diameter of Fe3 04 magnetic nanoparticles was 12 nm. The nanoparticles of different concentrations could enter the HaCaT cells by phagocytosis. After entering the cells the particles were released from phagocytic vesicles and exerted influence on the nearby mitochondria, leading to mitochondria swelling and cristae dissolving. Conclusion Fe3O4 magnetic nanoparticles can damage the ultrastructure of mitochondria near the particles in HaCaT cells, and the effect is in a concentration-dependent manner.