替米沙坦及吡哆胺对自发性高血压大鼠脑组织氧化应激的影响

Effect of telmisartan and pridoxamine on oxidative stress in brain tissue of spontaneously hypertensive rats

目的:探讨替米沙坦及吡哆胺对自发性高血压大鼠脑组织氧化应激的影响。方法:自发性高血压大鼠24只随机分为4组(n=6):高血压对照组(HC组);替米沙坦组(T组);吡哆胺组(P组);联合治疗组(TP组)。同龄WKY大鼠作为正常对照组(NC组)。药物干预16周,测定各组脑组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性及烟酰胺腺嘌呤二核苷酸磷酸(NADPH)氧化酶p47phox mRNA表达。结果:与NC组比较,HC组脑组织中MDA含量明显升高、SOD活性明显减低(P<0.05);与HC组比较T组、P组、TP组MDA含量明显减低,SOD活性明显升高(P<0.05);与NC组比较HC组(NADPH)氧化酶p47phox mRNA表达显著上调(P<0.01);与HC组比较T组、TP组NADPH氧化酶p47phox mRNA表达明显下调(P<0.01);HC组与P组比较NADPH氧化酶p47phox mRNA表达无统计学差异(P>0.05)。结论:自发性高血压大鼠脑组织处于氧化应激状态,替米沙坦及吡哆胺可抑制自发性高血压大鼠脑组织的氧化应激水平,联合治疗并不优于替米沙坦单药治疗。

Objective： To explore the effect of telmisartan and pridoxamine on oxidative stress in brain tissue of spontaneously hypertensive rats. Methods： Twenty-four spontaneously hypertensive rats were randomly divided into four groups（ n = 6） ： hypertensive control group （HC group）, telmisartun group（T group ）, pridoxamine group（P group ）, telmisartan and pridoxamine group（TP group）. Wistar-Kyoto （WKY） rats of the same age were served as a normal control group（NC group）. Drug treatment lasted for 16 weeks the level of malondialdehyde（MDA） in rat brain tissue, as well as superoxide dismutase（SOD） activity and nicotinamid adenine dinucleotide phosphate（NADHP） oxidaze p47phox mRNA expression were observed in this study. Results： The MDA level in brain in HC group were higher than that in NC group and the SOD activity were lower （ P 〈0.05）. T group, P group and TP group had lower MDA level and higher SOD activity than HC group （ P 〈 0.05）. The NADPH mRNA in brain in HC group were significantly higher than that in NC group（ P 〈 0.01）. T group and TP group had decreased levels of NADPH mRNA（ P 〈 0.01 ）, there was no significant difference between HC group and P group（ P 〉 0.05）. Conclusion： The brain tissue of spontaneous hypertensive rats had been under the status of oxidative stress. Single application of either telmisartan or pyridoxamiue could inhibit oxidative stress of brain tissue. However, compare with single treatment of telmisartan, no beneficial effects were observed in combined use of telmisartan and pridoxamine.