评价新型稳定同位素赖氨酸标记在定量蛋白质组学中的应用

Evaluation of isotopic labeling of lysine residues of peptides for quantitative proteomics

为了评价基于2-甲氧基-4,5-二氢-1氢-咪唑稳定同位素试剂在定量蛋白质组学中的应用价值,合成了轻型(D0)和重型(D4)的2-甲氧基-4,5-二氢-1氢-咪唑,通过对标准蛋白BSA酶解后产物的标记确认标记反应的特异性,并观察了标记物在MALDI-TOF-MS和LC-ESI-MS中定量的准确性,标记肽在串联质谱中的离子特点,以及对反相液相色谱行为的影响。结果表明,2-甲氧基-4,5-二氢-1氢-咪唑只与酶解后的肽段赖氨酸侧链氨基反应,具有良好的标记特异性;差异表达蛋白的定量可以通过MALDI和ESI电离模式实现;标记肽的串联质谱主要产生y离子,测序更为简便;反相液相色谱可以保持较好的分离效果,氘原子的引入不会影响保留时间,侧链修饰可以用于涉及液相色谱分离的蛋白质组学技术。2-甲氧基-4,5-二氢-1氢-咪唑稳定同位素试剂可以用于定量蛋白质组学。

To evaluate the reagent 2-methoxy-4,5-dihydro-lH-imidazole used for isotopic labeling in quantitative proteomics, we synthesized 2-methoxy-4,5-dihydro-lH-imidazole and its tetradeuterated analog in three steps. Prior to tryptic cleavage, bovine serum albumin （BSA） was reduced and alkylated. Tryptic peptides were derivatized with an equal volume of either DO or D4 and D4-derivatized peptides were mixed with at variable ratio （from 10：1 to 1：5） prior to MS and MS/MS analysis. We used Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry （MALDI-MS） and Electro Spray Ionization-Mass Spectrometry （ESI-MS） to evaluate the quantitative capability of labeling. The specificity of the reagent is excellent： only lysine side chains were modified among tryptic peptides. MALDI and ESI ionization modes not only could achieve the quantification of differentially expressed proteins but also facilitate the de novo sequencing. This side-chain modification can be used for quantitative analysis with proteomic strategies involving liquid chromatography. Reverse phase liquid chromatography （RPLC） kept a good resolution, and the introduction of D atoms did not introduce a variation of retention time between heavy and light peptides in RPLC.