坐骨神经阻滞对后足切割大鼠脊髓中脑源性神经营养因子表达的影响

Effect of sciatic nerve block on brain derived neurotrophic factor (BDNF) in the spinal cord following hindpaw incision in rats

目的探究坐骨神经阻滞能否影响大鼠后足切割后脊髓背角中脑源性神经营养因子(BDNF)表达的升高。方法雄性SD大鼠32只,体重150～250g,随机分为两组,各16只。坐骨神经阻滞组大鼠先行坐骨神经阻滞后后足切割手术,全麻组在全麻下行后足切割手术。两组均于术后1h、6h、1d、3d各取4只大鼠处死,检测腰段脊髓背角中BDNF的水平。结果后足切割后1h、6h、1d,全麻组大鼠切割侧腰段脊髓背角中BDNF水平显著高于对侧(P<0.05),坐骨神经阻滞组大鼠切割侧腰段脊髓背角中BDNF水平未见明显变化(P>0.05),两组大鼠切割后1h、6h、1d切割侧腰段脊髓背角中BDNF水平间比较差异有统计学意义(P<0.05)。结论坐骨神经阻滞能显著抑制大鼠后足切割引起的腰段脊髓背角中BDNF的表达升高。

Objective To investigate the effect of sciatic nerve block on the level of brain derived neurotrophic factor（BDNF） in the spinal cord following hindpaw incision in rats.Methods Thirty-two male S-D rats weighing 150～250 g were randomly divided into two groups,with 16 rats in each group.The rats in Group A were hind-paw incised following sciatic nerve block,and those in group B were hindpaw-incised under general anesthesia.Four rats in each group were randomly selected and sacrificed 1 h,6 h,1 d,3 d after the operation.The levels of BDNF were detected in their spinal cord.Results In group B,the levels of BDNF in the ipsilateral lumbar spinal cord was unregulated 1 h,6 h,1 d after hindpaw incision（P〈0.05）.In group A,the levels of BDNF in the lumbar spinal cord showed no significant change during the experiment（P〉0.05）.Statistically significant difference was found in the levels of BDNF in ipsilateral lumbar spinal cord 1 h,6 h,1 d after incision between the two groups.Conclusion Sciatic nerve block can significantly restrain the upregulation of the levels of BDNF in the lumbar spinal cord following hindpaw incision in rats.