两种流式细胞仪检测T细胞亚群的比对分析

The comparison of two types of flow cytometry on detection of T lymphocyte subsets

目的由于在测定全血T细胞亚群中CD3、CD4、CD8比例实验室可以使用BD与Beckman 2台流式细胞仪,为了保证两台仪器测定能提供一致的结果,于是对这两台仪器测定全血中CD3、CD4、CD8比例进行了比对。方法美国临床实验室标准化委员会提供了一套方案,来确保不同仪器测定能提供一致的结果。采用荧光免疫和流式细胞术相结合,新鲜肝素抗凝血,在两台仪器双份重复测定。首先检查方法内两次重复测定的离群点,然后检查仪器间离群点,随后检查方法的测定范围是否足够宽,最后是将预期偏差与允许偏差相比较。结果 CD3、CD4、CD8均未超过可允许偏差。结论实验室不用建立BD FACScan的正常参考值范围。

Objective For detection on proportion of CD3,CD4,and CD8 in the T lymphocytes subsets,laboratory may use two types of flow cytometry including BD and the Beckman.In order to guarantee two instruments determinations with consistent result,we compare the proportion of CD3,CD4 and CD8 in blood by two types of flow cytometry.Methods NCCLS has provided a scheme to guarantee the mensuration on different instrument with consistent result.We used the fluoroimmunoassay with flow cytometry,and the sample was flesh blood of patients.We inspected the outlier of repeated measures and outlier between the two types of equipment;Then we checked wether the range of measurement was wide enough.Finally we compared the anticipated deviation and allowable deviation.Results The deviation of CD3,CD4,CD8 was not over the controlling limit.Conclusion The laboratory should not establish the normal reference value scope for BD FACScan.