中山地区血小板供者HLA基因分型资料库的建立

Establishment of platelet donor registry with known HLA typings in Zhongshan area

目的建立血小板供者HLA基因分型资料库,探讨HLA-A、B基因频率及单体型频率,为临床患者提供HLA匹配的血小板,解决血小板输注无效(PTR)患者的血小板输注。方法采用聚合酶链反应-序列特异寡核苷酸探针杂交技术(PCR-SSOP)对568名中山地区单采血小板捐献者进行HLA-I类(A、B位点)基因分型并形成数据库,根据HLA表型群体资料,使用最大数学预期值算法(EM)计算HLA-A、B单体型频率。结果 HLA-A位点共检出17种等位基因,HLA-B位点共检出29种等位基因,单体型数153种。结论血小板供者HLA分型资料库的建立可以为PTR患者迅速寻找到HLA相合血小板,提高血小板输注效果。

Objective To establish a voluntary apheresis platelet donor registry with HLA typings in Zhongshan and study the allelic frequencies and haplotype frequencies of HLA-A,B,In order to resolve the platelet transfusion refractoriness（PTR）.Methods HLA class Ⅰ of 568 regular apheresis platelet donors in Zhongshan region were genotyped by PCR-SSOP and haplotype frequencies were calculated by computer software named Arleguin which was based on Expectation-Maximization（EM） algorithms.Results The number of alleles identified was 17 for HLA-A,29 for HLA-B,and 153 for haplotype respectively.Conclusion The establishment of voluntary apheresis platelet donors registry with HLA typings can rapidly find compatible donors for patients with platelet transfusions refractoriness（PTR）,and improve the effect of platlet transfusion.