蜻蜓凤梨花发育相关B类MADS-box基因克隆及表达分析

Clonging and Expression Analysis of B Function MADS-box Genes Involved in Floral Development from Aechmea fasciata

为探索MADS-box基因在凤梨花发育过程中的调控机制,通过设计简并引物,利用RACE技术,从蜻蜓凤梨花蕾中分离得到2个花发育相关B类MADS-box基因,分别命名为AfAP3和AfPI;AfAP3cDNA全长957bp,编码区编码226个氨基酸;AfPI cDNA全长808bp,编码区编码198个氨基酸,二者均具有典型的植物MADS-box蛋白结构.RT-PCR分析结果表明,AfAP3和AfPI基因主要在花器官中表达,在根系中也有微量表达;乙烯诱导后7d,AfPI基因在茎尖处开始有表达,表明此时蜻蜓凤梨花芽分化可能已经完成,AfAP3基因表达晚于AfPI.

Two flowering-related MADS-box genes, named as AfAP3 and AfPI were cloned from flower bud of Aechmea fasciata through designing degenerate primer and RACE technology. The full cDNA frag- ment of AfAP3 was 957 bp and coded a polypeptide of 226 amino acids. The full cDNA fragment of AfPI was 808 bp and coded a polypeptide of 198 amino acids. Both genes had a typical MADS-box domain. RT- PCR analysis showed that these two genes were main expressed in floral organs;on the other hand, they were also expressed a little in root. After flower induction by exogenous ethylene,AfPI gene began to ex- press in stem tip at 7 d,AfAP3 gene began to express later.