摘要
以山西省主栽苦荞品种‘黑丰1号’温室盆栽幼苗为材料,设置土壤活性炭含量分别为0(CK)、2.5(B2.5)、5.0(B5.0)、7.5(B7.5)、10(B10)g/kg共5个水平,研究土壤中施加活性炭后对苦荞幼苗根系及碳氮代谢、保护酶活性等指标的影响.结果显示:(1)随着活性炭施用比例的增加,苦荞幼苗根系生长指标和根系活力指标均呈先增后减的趋势,根平均直径呈先减后增的趋势,其中B5.0、B7.5处理的幼苗根系总长度、总表面积、总体积、活跃吸收面积、根尖数均显著高于对照,但B10处理的根系发育减弱.(2)随活性炭施用比例的增加,苦荞幼苗叶片蔗糖酶活性变化呈先增后减的趋势,同一处理水平条件下随苦荞的生长而逐渐下降;B2.5、B5.0处理苦荞幼苗叶片蔗糖酶活性和可溶性糖含量均比CK极显著增加,B7.5处理略有提高,B10处理差异不显著.(3)苦荞幼苗叶片谷氨酰胺合成酶(GS)活性随活性炭的增加基本呈上升趋势,而同一处理水平下随苦荞的生长而下降;叶片GS活性在B5.0、B7.5处理时比CK极显著提高,可溶性蛋白质含量在B7.5处理时也显著提高.(4)叶片保护酶SOD、POD、CAT活性随活性炭浓度的升高呈先升后降的变化趋势,而同一处理水平下各时期间变化不大;B2.5处理叶片的SOD、POD和CAT活性比对照显著增强.研究发现,适量施用活性炭(2.5~7.5g/kg)能有效促进苦荞幼苗碳氮代谢和保护酶活性,增强其根系活力.
In order to study the effects of activated carbon on the roots growth,C and N metabolism and protective enzymes of 'Heifeng No.1' in Shanxi,various doses of activated carbon(0,2.5,5.0,7.5 and 10 g/kg) were added into soil in greenhouse pot.Results showed that:(1)With the concentration of activated carbon increasing,the roots growth showed an optimal performance and the average diameter showed a minimum value;Total root length,total surface areas,total volume,root active absorbing areas and the number were improved significantly(P0.05) with 5.0(B5.0) and 7.5(B7.5) g/kg activated carbon applied,but the root grew weak with 10 g/kg activated carbon(B10).(2)With the concentration of activated carbon increasing,the activity of invertase reached a peak at B5.0,and then decreased gradually in pot culture;Invertase activity and soluble sugar content were increased significantly(P0.01) treated by 2.5(B2.5) and 5.0(B5.0) g/kg activated carbon,and B7.5 and B10 performed similar to the control(CK).(3)With the concentration of activated carbon increasing,the activity of GS increased and then decreased gradually in culture;The activities of GS were increased significantly(P0.01) treated by 5.0(B5.0) and 7.5(B7.5) g/kg activated carbon;and protein content at B7.5 was improved as well.(4)With activated carbon added increasing,the activities of SOD,POD and CAT also got a maximum and then changed very little with the culture time;The activities of SOD,POD and CAT at B2.5 were much higher than that of CK.Therefore,we concluded that certain activated carbon could improve the metabolism,activities of protective enzymes and root growth.
出处
《西北植物学报》
CAS
CSCD
北大核心
2012年第5期956-962,共7页
Acta Botanica Boreali-Occidentalia Sinica
关键词
苦荞
活性炭
根系生长
碳氮代谢
保护酶
Tartary buckwheat
activated charcoal
roots growth
C and N metabolism
protective enzyme