胰腺星状细胞超微结构的在体实验研究

Ultrastructural study of pancreatic stellate cells in experimental pancreatic fibrosis

目的在慢性胰腺炎动物模型，观察在体胰腺星状细胞的超微结构特点。方法Sprague-Dawley大鼠随机分为实验组（n=12）和对照组（n=12）。实验组给予16周高脂饮食诱导建立大鼠胰腺纤维化动物模型，对照组常规喂养。用HE和天狼猩红饱和苦味酸染色法观察胰腺组织病理学改变，免疫组化法识别胰腺组织中活化态及非活化态胰腺星状细胞，之后进一步通过透射电镜和免疫电镜方法对胰腺星状细胞的超微结构进行观察，最终对周细胞和泡心细胞进行形态学鉴别。结果病理形态学观察表明，长期高脂饮食可以诱导大鼠胰腺组织发生纤维化改变。实验组胰腺组织中活化态与非活化态胰腺星状细胞共存，细胞数量都较正常组明显增加。结论胰腺星状细胞在体超微结构特征的研究将为进一步研究其功能奠定形态学基础。

Objective To investigate the morphological and ultrastructural characteristics of pancreatic stellate cells （PSCs） in a chronic pancreatitis murine model. Methods Sprague-Dawley rats were divided into experimental group （n= 12） and control group （n= 12）. The experimental group was fed with a high-fat diet （HFD） for 16 weeks whereas the control group received regular rat feed. Pathologic changes in the pancreas were observed by several morphological studies including a Sirius Red staining method, as well as immunostaining for desmin and a smooth muscle actin （a-SMA）. TEM and immunoelectron microscopy were performed to study the ultrastructure of the PSCs. Results The number of desmin and a-SMA positive cells significantly increased in the HFD groups compared to the control group. The quiescent and activated PSC cells both coexisted in the pancreas in the HFD group. Conclusions The morphology and ultrastructural study of pancreatic stellate cells may be help- ful for further functional studies on chronic pancreatic fibrosis.