不同新生牛血清对体外培养BHK-21细胞的影响

Effect of various types of newborn bovine sera on culture in vitro of BHK-21 cells

目的比较不同新生牛血清对体外培养的BHK-21细胞的影响。方法以不同厂家和同一厂家不同批次的共6种新生牛血清培养BHK-21细胞,观察细胞传代后在不同血清培养条件下的形态、增长倍数和细胞维持时间。结果 6号血清培养的细胞生长状态最好,增长倍数最高,维持细胞存活时间最长;3号血清培养效果次之;2号、4号和5号血清培养的细胞至第4天仍未长满单层;1号血清培养的细胞基本未生长。结论不同厂家和同一厂家不同批次的新生牛血清对BHK-21细胞体外培养的效果存在差异。

Objective To compare the effect of various types of newborn bovine sera on culture in vitro of BHK-21 cells. Methods BHK-21 cells were cultured with six kinds of newborn bovine sera, manufactured by various manufacturers or of different batches manufactured by the same manufacturer, respectively, and observed for morphology, increased fold in quantity, and survival time after subculture. Results The cells cultured with serum No. 6 grew well, of which the increased fold in quantity was high and survival time was long as compared with those cultured with other newborn bovine sera. The culture efficacy of cells with serum No. 3 was only second to that with serum No.6. However, no monolayer was formed in the cells on day 4 after culture with sera No. 2, 4 and 5, and little growth was observed in the cells cultured with serum No.1. Conclusion Significant differences were observed in culture efficacies of BHK-21 cells with newborn bovine sera manufactured by various manufacturers or of different batches manufac- tured by the same manufacturer.