GnRH-a控制性超促排卵(COH)对小鼠着床期Hoxa-10基因表达的影响

Effect of GnRH-a Controlled Ovarian Hyperstimulation(COH) on Homeobox a10(Hoxa-10) Gene Expression in Mouse Peri-implantation

目的:研究GnRH-a长方案控制性超促排卵(COH)对小鼠妊娠率、胚胎着床率及着床期HOXA-10基因表达的影响,探讨COH影响胚胎着床的机制。方法:实验动物随机分为实验组(GnRH-a+hMG+hCG)和对照组(生理盐水),雌、雄合笼后观察小鼠阴栓率,于着床期取小鼠子宫,观察妊娠率及胚胎着床数,计算着床率;应用RT-PCR、Real-time PCR方法及免疫组织化学法分别检测小鼠着床期子宫内膜Hoxa-10 mRNA及蛋白的表达。结果:实验组小鼠阴栓率及妊娠率均明显低于对照组(P<0.01),实验组胚胎着床数高于对照组(P<0.01),但胚胎着床率较对照组显著降低(P<0.05),着床期小鼠子宫内膜Hoxa-10mRNA及蛋白表达下降,与对照组比较均有明显差异(P<0.05)。结论:GnRH-a长方案COH可引起小鼠着床期子宫内膜Hoxa-10表达下降,导致子宫内膜容受性降低,从而影响小鼠妊娠及胚胎着床。

Objective： To research the impact of long GnRH-a protocol controlled ovarian hyperstimulation （COH） on mouse pregnancy rate, embryo nidation rate, mRNA and protein expression of Hoxa-10 in peri-implantation, and to discuss the possible mechanisms of COH influencing on embryo nidation. Methods： Mice were randomly divided into treatment group （GnRH-a ＋hMG＋hCG） and control group （9 g/L, NaC1）, female and male mice were copulated, the situation of vaginal plug, pregnancy and embryo nidation were observed. RT-PCR, Real-time PCR were used to detect the concentration of Hoxa-10mRNA and immunohistochemical method was used to detect the concentration of HOXA-10 protein expression in the uterine tissue in peri-implantation. Results： The vaginal plug rate and pregnancy rate in treatment group were significantiy lower than those in the control （P〈0.01）, the number of the embryo nidation was larger in treatment group than that in the control （P〈0.01 ）, but the implantation rate of embyro was significantly lower in in treatment group than that in the control （P〈0.05）. Both PCR and immunohistochemical method showed that the expressions of HOXAIO mRNA and protein in treatment group were significantly lower than those in the control. Conclusion： The GnRH-a COH can decrease the expression of Hoxa-10, it disturbs the endometrial receptivity and window phase of normal uterus, thus lower the rate of pregnancy and embryo nidation.