凋亡相关分子血清可溶性Fas与不完全川崎病的关联研究

Study on the association of apoptosis-related molecule serum-soluble Fas with incomplete Kawasakidiseases

目的观察急性期川崎病（KD）、不完全川崎病（IKD）患儿和正常对照组3组之间的血清可溶性Fas（sFas）的表达水平，探讨sFas与IKD患儿的关联作用。方法选取本院住院治疗的急性期KD和急性期IKD患儿各32例，正常对照组20例，各组采用双抗夹心酶联免疫吸附试验（ELISA）检测血清sFas的含量，并行超声心动图检查，比较其sFas与IKD患儿的关联作用。结果（1）急性期KD组和急性期IKD组血清sFas水平高于对照组[（0．54±0．20）．g／L、（0．55±O．16）．g／LVS（0．24±0．04）ng／L，P〈0．05]；急性期KD组和急性期IKD组sFas水平高于对照组（F=29．276，P〈0．05）。（2）急性期IKD组超声心动图异常组与超声心动图正常组血清sFas水平比较差异有统计学意义[（0．65±0．19）ng／LVS（0．49±0．10）ng／L，t=3．139，P〈0．05]。KD组、IKD组sFas与PT-D、D-D、CRP、WBC均呈正相关（r=0．353—0．693，P〈0．05），与PLT无关（P〉0．05）。结论急性期IKD患儿的外周血清sFas水平呈高表达，其与急性期IKD患儿的心血管损害关系密切相关。

Objective To compare the levels of sFas in the sera among Kawasaki disease （KD）, incomplete Kawasaki disease （IKD）, and normal control groups, and to analyze the relationship of sFas with IKD children. Methods A total of 32 cases of acute KD and acute IKD children,and 20 cases of the control children were selected, respectively. The levels of serum sFas among three groups were measured u- sing ELISA kits. Each child among the three groups was examined by eehocardiography. Results （1）The levels of serum sFas among the three groups were [ （ 0. 54 ± 0. 20 ） ng/L in KD, （ 0. 55 ± 0. 16 ） ng,/L in IKD, and （0. 24 ± 0. 04） ng/L] in control group, respectively. The overall means of sFas in the KD and IKD groups were higher than the control group, and the differences were statistically significant（ F = 29. 276, P 〈 0. 05 ）. （2）The levels of serum sFas among echocardiography abnormal and normal groups were[ （0. 65 ±O. 19 ）ng/L and （0. 49 ±0. 10）ng/L ], respectively; and the difference between two groups were statistical- ly significant （ t = 3. 139, P 〈 0.05 ）. Conclusions The expression levels of sFas in the peripheral serum of IKD children were increased, and there was a close association of overexpression of sFas with the cardio- vascular damage in IKD children.