摘要
目的探讨自分泌运动因子受体（autocrine motility factor receptor，AMFR）在甲状腺乳头状癌中的表达情况，以及其与临床的关系。方法采用实时荧光定量PCR和免疫组化的方法对甲状腺乳头状癌进行研究。结果甲状腺乳头状癌和甲状腺正常组织在AMFR的mRNA和蛋白水平差异均有统计学意义（6．296±1．568v87．913±2．351，t=3．681，P=0．001；63．1％V834．5％，X^2=13．722，P〈0．001）。免疫组化分析显示甲状腺乳头状癌中AMFR蛋白的水平与肿瘤大小（X^2=5．209，P〈0．05）和淋巴结转移有关（X^2=4．32，P〈0．05），而与性别（X^2=0．739，P=0．39）、年龄无关（X^2=0．064，P=0．81）。结论AMFR在甲状腺乳头状癌中表达升高，其可能成为甲状腺乳头状癌治疗中的一个新靶点和预后的新指标。
Objective To investigate the expression of autocrine motility factor receptor （AMFR） in papillary thyroid carcinoma and its relationship with clinical characteristics of this disease. Methods Real - time quantitative PCR and immunolaistochemical methods were used to analyze the expression of AM- FR in papillary thyroid carcinomas. Results The significant differences in AMFR expression between pa- pillary thyroid carcinoma and normal thyroid tissues were found in the levels of mRNA （6. 296 ± 1. 568 vs 7. 913 ± 2. 351, t = 3. 681, P = 0. 001 ） and protein （63.1% vs 34. 5%, X^2 = 13. 722, P 〈 0. 001 ）, re- spectively. Immunohistochemistry analyses showed that the protein expression of AMFR in papillary thyroid carcinomas were significantly correlated with tumour size （ X^2 = 5. 209, P 〈 0. 05 ） and lymph node metasta- sis（X^2 =4. 32, P 〈0. 05）, and it was affected by the factors age（X^2 =0. 739, P =0. 39） and gender （X^2 =0. 064, P =0. 81）. Conclusions The increased AMFR in papillary thyroid carcinoma would be a new target for cancer therapy and a new marker for prognosis.
出处
《中国医师杂志》
CAS
2012年第6期750-752,755,共4页
Journal of Chinese Physician
