细胞视黄醇结合蛋白1在大鼠心肌梗死后肺重构中的作用

The role of cellular retinal-binding protein-1 on pulmonary structural remodeling after rat myocardial infarction

目的检测大鼠心肌梗死后不同时期肺组织中细胞视黄醇结合蛋白1（CRBP-1）的表达，以观察其在肺组织重构中的作用。方法通过结扎左冠状动脉前降支获得大鼠心肌梗死模型，60只成年雄性Wistar大鼠分为假手术组10只，术后6d处死取肺组织。心肌梗死组50只，分别于术后3、6、15、30和45d处死大鼠取肺组织。测量肺组织的重量，肺组织经处理后制成石蜡切片，苏木精-伊红、Masson’strichrome染色以及兔抗CRBP-1抗体免疫组织化学染色。图像分析系统检测肺组织中胶原的含量及CRBP-1阳性细胞数。结果39只大鼠存活并成功建立心肌梗死模型。大鼠心肌梗死后30d肺组织肺泡间隔增厚，胶原含量比假手术组明显增加（8．4％±3．6％比4．5％±2．6％，P〈0．001），心肌梗死后45d胶原含量与其他各组比较均明显增加（25．4％±8．8％，均P〈0．001）。梗死后3d肺泡间隔细胞即有CRBP．1阳性表达，15d时CRBP-1阳性细胞表达最高（43．8±7．4），此后逐渐较少，至45d时与梗死3d组数量相近（16．6±2．2比17．4±6．0，P〉0．05）。结论大鼠心肌梗死后肺组织结构重构，CRBP-1时序性表达参与了肺组织重构的过程。

Objectives To observe the expression of cellular retinol-binding protein-1 （ CRBP-1 ） in pulmonary tissues after rat myocardial infarction （MI） and uncover the role of CRBP-1 on the pulmonary structural remodeling. Methods MI was produced in male Wistar rats by left coronary ligation. Rats were sacrificed to obtain the lung at the 3^rd, 6^th, 15^th, 30^th, and 452 day after operation. After weighted, the rat lungs were fixed in 4% formalin and embedded in paraffin. Sections were cut and stained with hematoxylin and eosin （HE）, Masson＇s trichrome （MT）, rabbit anti-CRBP-I antibody. Results Thirty-nine Wistar rats survived and developed MI. Pulmonary tissue sections with HE and MT staining showed a remarkable lung structural remodeling. The content of pulmonary tissue collagen at the 30^rd was higher than in the sham group （8.4% ±3.6 vs 4. 5% ±2. 6, P 〈0. 001 ）. CRBP-1 expression was detected on the alveolar septa at the3rd day after operation, and peaked at the 15th day （43.8±7.4）, Then the CRBP-lexpression decreased, and arrived at the level of the 3^rd day. Conclusions We demonstrate that CRBP-1 is expressed temporal on the alveolar septa after rat MI. It indicates a potential relationship between CRBP-1 and lung structure remodeling process after MI.