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Tenascin-C在人原发性肝细胞癌浸润、转移和血管生成中的作用 被引量：5

Role of Tenascin-C in Invasion,Metastasis and Angiogenesis of Human Hepatocellular Carcinoma
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摘要 目的研究Tenascin-C在原发性肝细胞癌(HCC)中的表达,探讨Tenascin-C与HCC浸润、转移和血管生成的关系。方法采用免疫组织化学方法检测54例HCC、32例肝硬化、17例正常肝组织中Tenascin-C的表达情况以及微血管密度(MVD);采用逆转录聚合酶链反应(RT-PCR)方法检测Tenascin-C mRNA,并结合临床病理指标进行分析。结果 Tenascin-C在HCC、肝硬化和正常肝脏组织中的阳性例数分别为34、6和0,其差异具有统计学意义(χ2=14.06,P<0.01);Tenascin-C在HCC组织内的表达强度明显高于肝硬化组织[(162.15±9.77)vs.(149.24±8.25),t=2.192,P<0.05]。HCC组织Tenascin-C mRNA的表达强度明显高于肝硬化组织[(0.593±0.110)vs.(0.138±0.089),t=2.894,P<0.05]。有包膜浸润的HCC组织中Tenascin-C表达量明显高于无浸润者(t=2.248,P<0.05),Edmondson-Steiner病理分级中HCC属Ⅲ～Ⅳ级的Tenascin-C含量明显高于Ⅰ～Ⅱ级者(t=2.698,P<0.05),HCC有转移的Te-nascin-C含量明显高于无转移者(t=2.785,P<0.01)。HCC组织中Tenascin-C表达与微血管密度(MVD)呈正相关(r=0.68,P<0.05)。获随访的46例(85.2%)HCC患者在随访期间(3～30个月,平均11.5个月),Tenascin-C阴性表达的患者中位生存时间长于Tenascin-C阳性表达的患者[(22.01±6.95)月vs.(15.08±7.06)月,P<0.05,log-rank test]。结论 Tenascin-C对HCC的浸润、转移和血管生成起着重要作用。Tenascin-C和MVD可以作为预测HCC浸润、转移的生物学指标。 Objective To investigate the expression of Tenascin-C in human hepatocellular carcinoma（HCC）and its role in HCC invasion,metastasis and angiogenesis.Methods Immunohistochemical staining was employed to detect the expression of Tenascin-C and microvessel density（MVD）in resected specimens of 54 cases of HCC,32 cases of hepatic cirrhosis and 17 cases of normal hepatic tissues.Reverse transcription polymerase chain reaction（RT-PCR）was used to detect Tenascin-C mRNA expression.Results The immunohistochemistry revealed that Tenascin-C was positively expressed in 34 cases of HCC and 6 cases of hepatic cirrhosis tissues,respectively（χ2=14.06,P0.01）.No Tenascin-C was detected in normal hepatic tissues.The immunoreactivity of Tenascin-C in HCC was significantly higher than that in the cirrhosis tissues[（162.15±9.77）vs.（149.24±8.25）,t=2.192,P0.05].The expression of Tenascin-C mRNA in HCC was also significantly higher than that in hepatic cirrhosis tissues[（0.593±0.110）vs.（0.138±0.089）,t=2.894,P0.05].There was a correlation between Tenascin-C expression and capsule invasion（t=2.248,P0.05）,Edmondson-Steiner grade（t=2.698,P0.05）as well as metastasis（t=2.785,P0.01）in HCC.Additionally,MVD of HCC tissues was significantly correlated to the Tenascin-C expression（r=0.68,P0.05）.Forty-six cases of HCC（85.2%）were followed up with a median period of 11.5 months（range 3-30 months）.The Tenascin C-negative patients showed longer median survival time than the Tenascin-positive patients[（22.01±6.95）months vs.（15.08±7.06）months,P0.05,log-rank test].Conclusion Up-regulation of Tenascin-C in HCC is possibly related to tumor invasion,metastasis as well as angiogenesis.Tenascin-C and MVD staining might be used to predict the metastatic and aggressive potential of HCC.
作者 尹传昌 廖晓峰 陈雯 郭跃清 易继林 李兴睿
机构地区 华中科技大学同济医学院附属同济医院普外科 华中科技大学同济医学院附属同济医院妇产科
出处 《华中科技大学学报（医学版）》 CAS CSCD 北大核心 2012年第3期278-283,共6页 Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金 湖北省自然科学基金资助项目(No.2006ABA117)
关键词 TENASCIN-C 原发性肝细胞癌 微血管密度 肿瘤转移 Tenascin-C hepatocellular carcinoma microvessel density tumor metastasis
分类号 R735.7 [医药卫生—肿瘤]
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共引文献20

同被引文献42

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引证文献5

二级引证文献21

华中科技大学学报（医学版）
2012年 第3期

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