密蒙花总黄酮清除自由基活性研究

The Radical Scavenging Activity of Flavonoids from Buddleja officinalis Maxim

为了解密蒙花总黄酮清除自由基的能力,利用紫外-可见分光光度法对比研究了密蒙花总黄酮对1,1-二苯基-2-苦基肼自由基(DPPH.)、羟基自由基(.OH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS+.)和超氧阴离子自由基(O2-.)的清除作用。结果表明,密蒙花总黄酮能有效清除DPPH.、.OH、ABTS+.和O2-.。当密蒙花总黄酮质量浓度达到4.8 mg/L时,对DPPH.、.OH、ABTS+.和O2-.清除率分别可达77.88%、35.42%、85.08%和56.91%。

In order to know the free radical scavenging effects of flavonoids from Buddleja officinalis Maxim, UV-Vis spectrum was used to study the scavenging effects of flavonoids from B. officirtalis Maxim on 1,1-diphenyl-2-picrylhydrazyl （ DPPH＋ ） , hydroxyl （· OH） , ABTS＋ , and superoxide（ O2- ） free radicals. The results showed that flavonoids of B. officinalis Maxim could be well used in scavenging DPPH· ,·OH, ABTS＋ and O2- When the concentration of the flavonoids concentration of B. officinalis Maxim reached 4.8 mg/L, the scavenging rates could reach 77.88% , 35.42% , 85.08% and 56.91% , respectively.