膀胱移行细胞癌组织中E2F3、C-myc和Bcl-2的表达及其与膀胱癌相关性研究

Study on the expression of E2F3,C-myc,Bcl-2 in tissue of bladder transitional cell carcinoma and their correlation

目的:探讨膀胱移行细胞癌(BTCC)组织及正常膀胱黏膜组织中E2F3基因及其下游相关基因C-myc和Bcl-2的表达情况,同时分析E2F3、C-myc和Bcl-2的表达与临床病理参数之间的关系及3个指标之间的相关性,初步揭示BTCC发生、发展的分子机制。方法:检测不同病理分期及组织分级的BTCC标本和正常膀胱黏膜中E2F3、C-myc与Bcl-2蛋白的表达情况,分析E2F3 mRNA在不同组织中的表达情况,探讨BTCC组织中E2F3、C-myc与Bcl-2蛋白的表达之间的关系及三者与临床病理参数之间的关系。结果:E2F3表达阳性率在膀胱移行细胞癌与正常膀胱黏膜中的差异有显著性(P<0.05),且与肿瘤分级和分期密切相关(P<0.01)。肿瘤组织中E2F3 mRNA阳性表达而正常黏膜中E2F3 mRNA呈阴性表达。C-myc及Bcl-2在移行细胞癌组及正常膀胱黏膜中的表达率均有显著性差异(P<0.01)。其中移行细胞癌组C-myc表达与病理分级、组织分期有关(P<0.01),Bcl-2的表达率只与病理分级相关(P<0.01)。结论:E2F3与膀胱癌的恶性程度密切相关,其不仅是膀胱癌的诊断与预后指标,而且为膀胱癌的基因靶向治疗提供理论依据。E2F3与靶基因C-myc和Bcl-2的表达密切相关,E2F3可能通过与C-myc及Bcl-2协同作用共同参与了膀胱移行细胞癌的发生、发展。

Objective： To investigate the expression of E2F3, C-myc and Bcl-2 protein in bladder transitional cell carcinoma （BTCC） tissue and normal bladder epithelial tissue,and disscuss the correlation among the expression of E2F3, C-myc, Bcl-2 protein and the re- lationship of these three factors and the biological behaviors of BTCC. Methods： Immunohistochemistry was used to detect the expression of E2F3 and relative factors （C-myc and Bcl-2） in BTCC and normal bladder mucosa. E2F3 mRNA was investigated using RT-PCR anal- ysis in fresh bladder tumor tissues and normal bladder mucosa. Results： The expression rate of E2F3 was higher than normal bladder mu- cosa（P〈0.05）, which was strongly correlatated with pathological grade and clinical stage（P〈0.01）. The positive expression of E2F3 mRNA in BTCC was 100% and in normal bladder mucosa was 0% .There was significant difference in the rates of the expression rate of C-myc and Bcl-2 in BTCC and normal bladder mucosa （P〈0.01）. The expression of C-myc in BTCC was strongly correlated with pathological grade and clinical stage （P〈0.01）. The expression of Bcl-2 in BTCC was only correlated with pathological grade （P〈0.01）. Conclusion： Results indicates that E2F3 is a diagnostic and prognostic index of BTCC, and it provides theory basis about the gene target therapy in BTCC. There is a close relationship between E2F3 and C-myc or Bcl-2 expression in BTCC tissue. So the cooperation of the three factors may participate in the carcinogenesis and progress of BTCC.