抗疏健骨颗粒对骨质疏松模型大鼠骨密度和骨生物力学的影响

Influence of Kangshu Jiangu Granules on Bone Density and Biomechanics of the Osteoporotic Model Rats

目的:观察抗疏健骨颗粒对骨质疏松模型大鼠骨密度和骨生物力学的影响,研究其防治骨质疏松症的作用机理。方法:60只大鼠喂养1周后随机分为假手术组(10只)和去势组(50只)。去势组随机分5组,模型组,尼尔雌醇组,抗疏健骨颗粒小、中、大剂量组,每组10只,除假手术组外,其余5组大鼠行卵巢切除术造模。抗疏健骨颗粒大、中、小剂量组分别给予抗疏健骨颗粒混悬液0.4 g/mL、0.2 g/mL、0.1 g/mL,2 mL/d,每日1次,尼尔雌醇组给予尼尔雌醇片(1 mg/kg体质量),配成0.15 g/L混悬液,1周1次,每次2 mL,模型组和假手术组以生理盐水(8 mL/kg体质量)灌胃。12周后测定离体骨密度。计算最大载荷、断裂挠度、弯曲能量。结果:与模型组比较,抗疏健骨颗粒大、中剂量组的腰椎骨和股骨远端骨密度均显著增加(P<0.01),抗疏健骨颗粒大、中剂量组与尼尔雌醇组的最大载荷显著增高(P<0.01),但小剂量组增加不明显(P>0.05);抗疏健骨颗粒各剂量组断裂挠度与模型组比较,虽有升高趋势,但变化不明显(P>0.05);尼尔雌醇组、抗疏健骨颗粒大剂量组的弯曲能量与模型组比较,增高显著(P<0.01),中、小剂量组虽有升高趋势,但无显著差异(P>0.05)。结论:抗疏健骨颗粒能够改善骨密度和骨生物力学性能,恢复骨代谢平衡,从而达到防治骨质疏松的作用。

Objective： To observe the influence of Kangshu Jiangu Granules on bone density and femoral biomechanics of the osteo- porotic model rats and study the mechanism of preventing and treating osteoporosis. Methods ： 60 rats fed after one week were ran- domly divided into sham operation group （10） and castration group （50）. Castration group were randomly divided into five smaller groups with ten rats in each group： model group, neal estradiol group, small, medium and large dose groups of Kangshu Jiangu Granules. Except the sham operation group, other 5 groups were made models with oophorectomy. The small, medium and large dose groups of Kangshu Jiangu Granules were respectively given suspension liquid of Kangshu Jiangu Granules 0. 4 g/mL, 0.2 g/mL, 0. 1 g/mL, 2 mL/d once a day. Neal estradiol group were given 0. 15 mg/mL suspension liquid of Neal estradiol （ 1 mg/kg weight） once a week and 2 mL each time. Model group and sham operation group were given gastrol perfuson of physio- logical saline （8 mL/kg weight） . 12 weeks later, the isolated bone density was measured to calculate the maximum load calcula- tion, the maximum deflection and bending energy. Results ： The bone density of the lumbar vertebrae and the disral end of the fe- mur of the small, medium and large dose groups of Kangshu Jiangn Granules were significantly increased （ P 〈 0.01 ） compared with those of the model group ; the maximum load in the group of the small, medium and large dose groups of Kangshu Jiangu Granules and neal estradiol group increased significantly （ P 〈 0.01 ）, but the increase in the group of small dose is not obvious （ P 〉 0.05 ） ; The crack deflection of all groups of Kangshu Jiangu Granules had an unobvious rise trend （P 〉 0.05 ） compared with the model group; the bending energies of neal estradiol group and the large dose groups of Kangshu Jiangu Granules increased significantly （P 〈0.01 ）compared with that of the model group; the medium and small dose group had an increase trend, but there was no sig- nificant difference （P 〉0.05）. Conclusion： Kangshu Jiangu Granules can improve the bone density and bone biomechanical per- formance, restore bone metabolism balance, so as to achieve the function of preventing osteoporosis.