维药买朱尼含药血清对IL-1β作用下大鼠软骨细胞MMP-13、Type-ⅡCollagen表达的影响

Effect of Weiyao-maizhuni containing serum against rat chondrocyte MMP-13,type-II collagen expression under the action of IL-1β

目的:观察维药买朱尼含药血清对IL-1β作用下体外培养的大鼠关节软骨细胞Type-ⅡCollagen、MMP-1、MMP-13表达的影响,并进一步探讨维药买朱尼防治OA的作用机制。方法:从1周龄SD大鼠关节软骨中分离培养原代软骨细胞,经鉴定后选用第二代细胞随机分为空白对照组、模型组、维药买朱尼组,培养第3d时模型组、维药买朱尼组采用细胞因子IL-1β(10ng/ml)继续培养,分别在培养后第24h、36h、48h采用RealTimePCR方法检测并分析各组软骨细胞中MMP-13、Type-ⅡCollagen的表达情况。结果:在第24h时各组MMP-13表达增加,Type-ⅡCollagen有一定降低,但无统计学差异;而在第36h和48h时空白对照组与模型组MMP-13、Type-ⅡCollagen表达差异均有统计学意义(P<0.05),维药买朱尼组较模型组MMP-13与Type-ⅡCollagen的表达在48h、72h时间点有显著性差异(P<0.05)。结论:维药买朱尼可抑制IL-1β对Type-ⅡCollagen的降解和破坏作用,并能抑制IL-1β对MMP-13的诱导和激活作用。

Objective：To investigate the effects of Weiyao-maizhuni medicated serum on the expression of MMP-13,Type-II collagen of rats articular chondrocytes stimulated by interleukin-1 beta.Methods：Articular chondrocytes were obtained from the cartilage of 1-week rats and cultured in vitro,the passage II chondrocytes were used.They were divided into 3 groups：Control groups,model groups,Weiyao-maizhuni groups.After 3 days cultured,IL-1 Beta 10ng/ml were added in the control groups,model groups and Weiyao-maizhuni groups.Real time PCR method was adopted to observe the influence of every different group on the expression of MMP-13 and type-II collagen of chondrocytes stimulated by interleukin-1 beta after incubated 24h,36h and 48h.Results：Groups of MMP-13 expression increased and the type-II collagen have some reduce in the first 24h,but not significantly;MMP-13,type-II collagen of control group and model group expression differences were statistically significant （P0.05） in the 36h and 48h;MMP-13 and type-II collagen expression between Weiyao-maizhuni group and model group has significant differences （P0.05）.Conclusion：Weiyao-maizhuni medicated serum can significantly inhibit the degradation and destroy of type-II collagen by IL-1β,at the same time can inhibit the IL-1β-induced MMP-13.