摘要
目的通过上调人肝癌HepG2细胞株中miRNA-101表达,探讨miRNA-101抑制靶基因EZH2对肝癌细胞增殖的影响。方法人工合成miRNA-101模拟体并转染至HepG2肝癌细胞株中,利用MTT法检测细胞增殖抑制、流式细胞仪检测细胞周期、实时荧光定量PCR检测EZH2 mRNA表达。结果 miRNA-101模拟体转染组HepG2细胞增殖活性明显低于阴性对照组及空白对照组(P<0.05),miRNA-101模拟体组G0/G1期细胞明显增多,为(74.71±4.6)%,S期细胞减少,为(14.57±1.1)%(P均<0.05);miRNA-101模拟体组EZH2 mRNA较阴性对照组和空白对照组表达下降(P<0.05)。结论上调miRNA-101可抑制EZH2基因表达,对人肝癌细胞增殖活性具有负调控作用。
Objective To investigate the effects of inhibiting enhancer of zeste homolog 2 （EZH2） on cell proliferation by up-regulating the expression of miRNA-101 in HepG2 cells. Methods Synthetic miRNA-101 mimics was transfected into HepG2 cell lines. The growth of transfected HepG2 cells was detected by methyl thiazolyl tetrazolium （MTI＇）. The cell cycle was tested by flow cytometry（FCM）. And the expression of EZH2 mRNA was detected by real-time qRT-PCR. Results The cell proliferative activity was significantly lower in the miRNA-101 mimics group than that in the negative control group and blank control group, respectively （all P〈 0.05 ）. In the miRNA-101 mimics group, cells increased significantly at G0/G1 phase [ （74.71±4.6） % ] while decreased at S phase [ （ 14.57±1.1 ） %, P 〈 0.05 ] ; And the expression of EZH2 mRNA was down-regulated （P 〈 0.05）. Conclusion Up-regulated miRNA-101 can inhibit the expression of EZH2, which plays a negative regulation role on cell proliferative activity of hepatic carcinoma.
出处
《广西医学》
CAS
2012年第6期667-669,688,共4页
Guangxi Medical Journal
基金
广西自然科学基金(0728153)
