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COS-7细胞株中KCNE2与KChIP2c共表达对Kv4.2电流复活的影响 被引量：1

The effect of co-expression of KCNE2 and KChIP2c on Kv42 current recovery from inactivation of COS-7 cells
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摘要 目的探讨当钾通道相互作用蛋白(KChIP2)和KCNE2共表达时对电压依赖性钾通道Kv4.2电流复活的影响。方法采用COS-7株细胞表达系统模拟生理条件,将Kv4.2、KChIP2c和KCNE2 cDNA以3∶1∶1的分子比同时转染至COS-7株细胞,用膜片钳的方法记录全细胞电流,用免疫组化聚焦实验和免疫荧光成像证实细胞表达cDNAs编码的蛋白质。结果 KChIP2c加速了Kv4.2的复活,KCNE2与KChIP2c共表达进一步加速了电流的复活,导致了在Kv4.2电流恢复中的"超射"峰电流,一种人类瞬间外向电流(Ito)特有的现象。结论 Kv4.2与KChIP2c和KCNE2的共表达可产生与人类天然Ito相似的电流,提示KChIP2c和KCNE2同时参与构成心肌细胞Ito的电生理特性。 Objective To study the effect of co-expression of KCNE2 and KChIP2c on voltage-dependent K＋ channel Kv4.2 current recovery from inactivation of COS-7 cells.Methods The expression of COS-7 cells was used to simulate physiological conditions.Kv4.2,KChIP2c and KCNE2 cDNA were simultaneously transfected into COS-7 cells at a molar ratio of 3：1：1.Whole-cell current was recorded by patch-clamp method.Proteins encoded by cDNAs were detected by immunocytochemical confocal experiment,and immunofluorescence was imaged with fluorescent microscopy.Results KChIP2c accelerated Kv4.2 recovery from inactivation.Co-expression of KCNE2 and KChIP2c further accelerated the recovery and caused an ＂over-shoot＂ of peak current amplitude during Kv4.2 current recovery,a phenomenon which has been uniquely described for human transient outward current（Ito）.Conclusion Co-expression of Kv4.2 with KChIP2c and KCNE2 yields a current profile similar to human native Ito.Both KChIP2c and KCNE2 simultaneously participate in recapitulation of electrophysiological properties of Ito in cardiomyocytes.
作者 王芳 吴红玲 凌云 张健 徐明明
机构地区 深圳市南山区人民医院VIP病房 深圳大学医学院生理学教研室
出处 《临床和实验医学杂志》 2012年第13期993-995,998,共4页 Journal of Clinical and Experimental Medicine
基金 深圳市南山区卫生科技计划项目基金资助(项目编号:2009017)
关键词 瞬间外向电流 钾通道相互作用蛋白 KCNE2 电生理 Transient outward current KChIP2 KCEN2 Electrophysiology
分类号 R363 [医药卫生—病理学]
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参考文献14

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二级参考文献11

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临床和实验医学杂志
2012年 第13期

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