猪瘟免疫猪接种PRRSV nsp2Δ1882-2241弱毒疫苗后IL-12、IL-10、TNF-α应答特征

Changes of IL-12,IL-10 and TNF-α in pigs inoculated by PRRSV vaccine after CSF vaccination

不同时间段采集猪的血液样本,利用ELISA方法检测血清和细胞培养上清中IL-12、IL-10、TNF-α等3种细胞因子的水平,分析猪双重免疫CSFV、PRRSV弱毒苗后血清和细胞培养上清中细胞因子的变化规律。结果显示,IL-12在血清中的浓度显著高于细胞培养上清中的浓度(P<0.01),在CSFV高抗体PRRSV高抗体组中28d采血的细胞培养上清中IL-12的浓度高于14d采血的细胞培养上清中的浓度(P<0.05);IL-10血清中水平显著低于细胞培养上清中水平(P<0.01),在CSFV高抗体PRRSV高抗体组中28d采血的细胞培养上清中的IL-10浓度显著低于14d采血的细胞培养上清中的浓度(P<0.01);TNF-α在血清中水平显著低于细胞培养上清中水平(P<0.01),在3组中28d采血的细胞培养上清液中TNF-α浓度高于14d采血的细胞培养上清液中浓度(P<0.05)。结果表明,2种疫苗特异性抗体应答高时,IL-12水平显著上调,IL-10水平显著下调,提示上调IL-12及下调IL-10都是基因缺失疫苗发挥效力的潜在机制。TNF-α总体水平上升,但在血清中水平较低。

The levels of IL-10, IL-12 and TNF-α in serum and cell culture supernatant were measured by ELISA, changes of cytokines in serum and cell supernatant were analyzed at different periods after vaccinated CSFV,PRRSV attenuated vaccine. The results showed that concentration of IL-12 in serum were significantly higher than in cell culture supernatant（P〈0.01）,in high CSFV- PRRSV antibody group,concentration of IL-12 in the cell culture super- natant were higher at 28 d than at 14 d （P〈0.05）. Levels of IL-10 in serum were significantly lower than in cell cul- ture supernatant （P〈0. 01） ,in high CSFV- PRRSV antibody group,concentration of IL-10 in cell culture supernatant were significantly lower at 28 d than at 14 d （P〈0.01）. Concentration of TNF-α in serum were significantly lower than the level in cell culture supernatant （P〈0.01）, the concentration of TNF-α in the culture supernatant were higher at 28 d than at 14 d in the three groups（P〈0.05）. These results indicated when the two vaccine-specific antibody response were high,IL-12 levels were significantly raised and IL-10 levels were significantly reduced, sug- gesting that increased IL-12 and down-regulating IL-10 are a potential mechanisms of gene-deleted vaccine. The over- all level of TNF-α in the supernatant was increased after two vaccines immunized,but low levels in serum.