白鸡屎藤挥发油抗肠炎沙门菌及其内毒素的作用

Anti-salmonella enteritidis and anti-endotoxin effect of volatile oil of white paederia scandens in broilers

雏鸡肠炎沙门菌病是肠炎沙门菌及其内毒素共同作用的结果。为了研究白鸡屎藤挥发油抗肠炎沙门菌及其内毒素的作用,本试验测定了白鸡屎藤挥发油抗肠炎沙门菌的效果,进一步提取了肠炎沙门菌内毒素并测试了白鸡屎藤挥发油对肠炎沙门菌内毒素的体外灭活作用和对肠炎沙门菌内毒素致热的解热作用,同时本试验还对白鸡屎藤挥发油进行了定性定量分析并测试了白鸡屎藤挥发油及其主要药理活性成分的药动学特征。结果显示,白鸡屎藤挥发油包括丁香酚、樟脑、龙脑、水杨酸甲酯等活性成分,在中剂量(100mL/L)、高剂量(150mL/L)均表现出较强的体外抑菌效果,25mL/L以上浓度即可表现出较强的灭活内毒素作用,并且2～4h大部活性成分进入血液循环,表现出显著的解热作用。结果表明,白鸡屎藤挥发油具有一定的抗菌、抗内毒素和解热作用,可以用于雏鸡肠炎沙门菌感染性发热病的治疗。

The anti-Salmonella enteritidis and anti-endotoxin effect of the white paederia scandens volatile oil. were e- valuated in broilers. Meanwhile, qualitative and quantitative analysis for white paederia scandens volatile oil were done to explore its main ingredients pharmacokinetic characteristics. The results showed that the white paederia scandens volatile oil including eugenol, camphor, borneol, methyl salicylate and other active ingredients showed in vitro inhibition bacterial effect in middle-dose （100 mL/L） and high-dose （150 mL/L）,the inactivated endotoxin above 25 mL/L the volatile oil, and a significant antipyretic effect at 2-4 h. These results suggest that the white paederia scandens volatile oil has anti-Salmonella enteritidis ,anti-endotoxin and antipyretic effects, and can be used for the treatment against Salmonella enteritidis in broilers.