摘要
以鲨鱼软骨为原料 ,用改进的提取工艺 ,制得纯度较高的鲨鱼软骨多糖(SCAMP)。对其进行理化性质分析 ,表明SCAMP含有29.90%N 乙酰半乳糖胺、27.00 %葡萄糖醛酸、13.50%硫酸根及0.20 %蛋白质。红外光谱测试表明SCAMP的糖残基可能是α -糖苷键 ,推测其主要成分是硫酸软骨素类多糖。用荧光探针法证实SCAMP与DNA分子能相互作用 ,并呈量效关系 ,为进一步探索SCAMP抗癌作用机理提供了一项实验依据。
In this paper, shark cartilage acid mucopolysoccaride (SCAMP) was extracted from shark cartilage by the improved method. The results of physiocochemical properies experiments showed that SCAMP is composed of 29.90% aminogalactose, 27.00% glucuronic acid, 13.50% SO2-4 and 0.20% protein. Frared spectra experiment suggested that all residues of SCAMP are linked by α glucosidic bond. By conclution, the most of SCAMP is likely chondroitin sulfate. By fluorescence probe method it is proved that SCAMP can interact with DNA, and the inhibitory rate was concentration dependent, which is an important proof to understand its preventive effect of cancer and anticancerous effect more.
出处
《海洋科学》
CAS
CSCD
北大核心
2000年第5期40-43,共4页
Marine Sciences
基金
江苏省自然科学基金资助项目
