摘要
为了研究地塞米松、油酸、乳酸对HSL mRNA表达的影响,试验采用实时荧光定量方法检测脂肪细胞中HSL mRNA的表达量。结果表明:油酸、乳酸抑制脂肪细胞内HSL mRNA表达,抑制作用随着浓度增加而增强;地塞米松促进脂肪细胞内HSL mRNA表达,促进作用存在剂量依赖性。
To investigate the effects of dexamethasone,oleic acid and lactic acid on the expression of HSL mRNA in adipocytes,the real-time fluorescent quantitative PCR was used to determine the expression levels of HSL mRNA in adipocytes.The results showed that the expression levels of HSL mRNA were inhibited in adipocytes treated with oleic acid and lactic acid,respectively.The inhibition increased with the increase of oleic acid and lactic acid concentrations,dexamethasone could promote the expression of HSL mRNA in adipocytes,and the effect of promotion was dose-dependent.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2012年第7期1-4,179,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(3023062)
"长江学者和创新团队发展计划"创新团队项目(IRT0848)
四川省教育厅重点资助项目(10ZA048)
四川农业大学优秀学士毕业论文培育计划项目
