绿头鸭卵黄硒含量与禽流感新城疫病毒抗体滴度关系探讨

Relationship between selenium content and antibody of AIV,NDV in mallards′yolk

绿头鸭(Anas platyrhynchos)是大庆龙凤湿地的优势物种,为研究绿头鸭卵黄硒含量与病毒抗体之间的相关关系,采用血凝抑制试验及原子荧光光谱法(AFS)检测105枚绿头鸭卵黄内硒含量及H1、H5、H9亚型禽流感病毒(AIV)和新城疫病毒(NDV)抗体。结果表明,卵黄硒含量为0.48μg/g±0.15μg/g;卵黄内H1、H5、H9亚型AIV和NDV抗体阳性率分别为:100%、59.05%、71.43%、68.57%;其评价抗体滴度分别为:6.17log2、2.89log2、3.34log2、4.22log2。经相关分析结果表明,卵黄内H1、H5、H9亚型AIV和NDV抗体滴度与卵黄硒含量的相关系数分别为:0.077、0.714、0.690、0.611。由此可见,龙凤湿地绿头鸭种群卵黄硒含量与相关病毒抗体之间呈明显的正相关,说明硒元素对动物机体抗病毒能力起到一定的增强作用。

The mallard（Anas platyrhynchos） is the dominant species in Daqing Longfeng wetland.In order to understand the correlation between antibody and selenium（Se） content in the mallards’ yolk,105 in-nest eggs of mallard were collected.The AIV and NDV antibodies in the yolk were tested using hemagglutination inhibition（HI）,and Se content were measured by atomic fluorescence spectrometry（AFS）.The results showed that Se content was 0.48±0.15 μg/g in the yolk;H1,H5,and H9 subtypes of AIV and NDV antibody positive rates were 100%,59.05%,71.43%,and 68.57%,respectively;their antibody titers were to 6.17log2,2.89log2,3.34log2,and 4.22log2,respectively.The correlation analysis showed that H1,H5,and H9 subtypes of AIV and NDV antibody titers and Se content of the correlation coefficients were 0.077,0.714,0.690,and 0.611,respectively.Therefore,the antibody titers of H5,H9 subtype AIV and NDV were extremely positively correlative to Se content in the mallards′ yolk,and Se had an important role in enhancement of anti-virus capability.