灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系分析

Analysis of genetic diversity and genetic relationships of Lonicera macranthoides cultivars

目的:研究灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系。方法:以灰毡毛忍冬的5个主栽品种为试材,采用ISSR和SRAP分子标记技术,利用Treeconw软件分析处理数据,UPGMA法聚类,构建亲缘关系系统图。结果:20条ISSR引物共得到186条扩增条带,其中有103条呈现多态性,占54.63%,遗传距离0.058 4～0.230 8,平均值为0.190 2。58个SRAP引物组合共得到591条扩增条带,其中有347条呈现多态性,占55.46%,遗传距离在0.107 1～0.261 1,平均值为0.212 2。2种标记均表明灰毡毛忍冬主栽品种的遗传多样性仅居中等水平。2种标记系统得到了相似但并不完全相同的聚类图。结论:灰毡毛忍冬主栽品种有中等水平的遗传多样性,ISSR与SRAP标记均适用于其遗传多样性的分析。

Objective： To study genetic diversity and genetic relationships among Lonicera macranthoides cultivars. Method： Five cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system dia- gram of genetic relationship was built by UPGMA. Result： Twenty ISSR primers amplified 186 bands with 103 （54.63%） polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347 （55.46%） polymorphic bands. Genetic distance ranges were 0. 058 44）. 230 8 （by ISSRs） and 0. 107 14）. 261 1 （by SRAPs）. Both ISSR and SRAP analyses revealed a middle level of genetic di- versity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same. Conclusion： The ge- netic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.