摘要
目的:研究灰毡毛忍冬主栽品种的遗传多样性及其亲缘关系。方法:以灰毡毛忍冬的5个主栽品种为试材,采用ISSR和SRAP分子标记技术,利用Treeconw软件分析处理数据,UPGMA法聚类,构建亲缘关系系统图。结果:20条ISSR引物共得到186条扩增条带,其中有103条呈现多态性,占54.63%,遗传距离0.058 4~0.230 8,平均值为0.190 2。58个SRAP引物组合共得到591条扩增条带,其中有347条呈现多态性,占55.46%,遗传距离在0.107 1~0.261 1,平均值为0.212 2。2种标记均表明灰毡毛忍冬主栽品种的遗传多样性仅居中等水平。2种标记系统得到了相似但并不完全相同的聚类图。结论:灰毡毛忍冬主栽品种有中等水平的遗传多样性,ISSR与SRAP标记均适用于其遗传多样性的分析。
Objective: To study genetic diversity and genetic relationships among Lonicera macranthoides cultivars. Method: Five cultivars were estimated by ISSR and SRAP. The data of amplified bands were analyzed by Treeconw software. The system dia- gram of genetic relationship was built by UPGMA. Result: Twenty ISSR primers amplified 186 bands with 103 (54.63%) polymorphic bands and 58 SRAP primer combinations amplified 591 bands with 347 (55.46%) polymorphic bands. Genetic distance ranges were 0. 058 44). 230 8 (by ISSRs) and 0. 107 14). 261 1 (by SRAPs). Both ISSR and SRAP analyses revealed a middle level of genetic di- versity in L. macranthoides cultivars. The dendrograms based on SRAP and ISSR markers were not all the same. Conclusion: The ge- netic diversity of L. macranthoides cultivars is middle. ISSR and SRAP markers can be effectively applied to genetic analysis in L. macranthoides cultivars.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第13期1912-1916,共5页
China Journal of Chinese Materia Medica
基金
国家“十一五”科技支撑计划项目(2006BAI06A12)
重庆市自然科学基金项目(CSTC2009BB5395)
重庆市重大科技攻关项目(CSTC2009AA5052)
重庆市重大科技专项(CSTC2009AA5009)